ID   RF1_MESFL               Reviewed;         363 AA.
AC   Q6F0I3;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=Peptide chain release factor 1 {ECO:0000255|HAMAP-Rule:MF_00093};
DE            Short=RF-1 {ECO:0000255|HAMAP-Rule:MF_00093};
GN   Name=prfA {ECO:0000255|HAMAP-Rule:MF_00093}; OrderedLocusNames=Mfl634;
OS   Mesoplasma florum (strain ATCC 33453 / NBRC 100688 / NCTC 11704 / L1)
OS   (Acholeplasma florum).
OC   Bacteria; Tenericutes; Mollicutes; Entomoplasmatales; Entomoplasmataceae;
OC   Mesoplasma.
OX   NCBI_TaxID=265311;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33453 / NBRC 100688 / NCTC 11704 / L1;
RA   Birren B.W., Stange-Thomann N., Hafez N., DeCaprio D., Fisher S.,
RA   Butler J., Elkins T., Kodira C.D., Major J., Wang S., Nicol R., Nusbaum C.;
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC       translation in response to the peptide chain termination codons UAG and
CC       UAA. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF1. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000255|HAMAP-Rule:MF_00093}.
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DR   EMBL; AE017263; AAT75990.1; -; Genomic_DNA.
DR   RefSeq; WP_011183530.1; NC_006055.1.
DR   RefSeq; YP_053874.1; NC_006055.1.
DR   AlphaFoldDB; Q6F0I3; -.
DR   SMR; Q6F0I3; -.
DR   STRING; 265311.Mfl634; -.
DR   EnsemblBacteria; AAT75990; AAT75990; Mfl634.
DR   GeneID; 2897719; -.
DR   KEGG; mfl:Mfl634; -.
DR   PATRIC; fig|265311.5.peg.636; -.
DR   eggNOG; COG0216; Bacteria.
DR   HOGENOM; CLU_036856_0_1_14; -.
DR   OMA; ISDHRVG; -.
DR   Proteomes; UP000006647; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00093; Rel_fac_1; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004373; RF-1.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00019; prfA; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..363
FT                   /note="Peptide chain release factor 1"
FT                   /id="PRO_0000177697"
FT   MOD_RES         237
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00093"
SQ   SEQUENCE   363 AA;  40688 MW;  BA27675EE2761829 CRC64;
     MNPKTYEALE TMQKRVDQID KDLQSEEIVS DVKKMLELNK ERANLIEVVE KFIEYKTIIQ
     SIADAKEILG NEKDAEMIEL AKMELSENED AVEPIVAIIE ELLLPKDPND DKNVIVEIRG
     AAGGDEANIF AGDLLRMYKL YAETQNWKIT MLDANSSEAG GFSQVSFMVK GDRVYSKLKF
     ESGAHRVQRV PKTEAKGRIQ TSTATVAVLP EMSDVEIEIK NSDLRIDTYR SSGAGGQHVN
     TTDSAVRITH IPTGVVAASQ DGRSQHDNKD IAMTMLRARI YEAELEKQQA EADATRKNAV
     GTGARSEKIR TYNYPQNRVT DHRVGLTLNK LDQVMEGKID DFIIALVNDE QRQKVEAQLQ
     DNE