ID   RF1_MYCCT               Reviewed;         363 AA.
AC   P71496; Q2SSX8;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 2.
DT   25-MAY-2022, entry version 128.
DE   RecName: Full=Peptide chain release factor 1;
DE            Short=RF-1;
GN   Name=prfA; OrderedLocusNames=MCAP_0144;
OS   Mycoplasma capricolum subsp. capricolum (strain California kid / ATCC 27343
OS   / NCTC 10154).
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX   NCBI_TaxID=340047;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8635731; DOI=10.1016/0378-1119(95)00791-1;
RA   Inagaki Y., Bessho Y., Hori H., Osawa S.;
RT   "Cloning of the Mycoplasma capricolum gene encoding peptide-chain release
RT   factor.";
RL   Gene 169:101-103(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=California kid / ATCC 27343 / NCTC 10154;
RA   Glass J.I., Lartigue C., Pfannkoch C., Baden-Tillson H., Smith H.O.,
RA   Venter J.C., Roske K., Wise K.S., Calcutt M.J., Nelson W.C., Nierman W.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC       translation in response to the peptide chain termination codons UAG and
CC       UAA. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF1 (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000305}.
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DR   EMBL; D50100; BAA08795.1; -; Genomic_DNA.
DR   EMBL; CP000123; ABC01154.1; -; Genomic_DNA.
DR   RefSeq; WP_011387040.1; NC_007633.1.
DR   AlphaFoldDB; P71496; -.
DR   SMR; P71496; -.
DR   EnsemblBacteria; ABC01154; ABC01154; MCAP_0144.
DR   GeneID; 23778903; -.
DR   KEGG; mcp:MCAP_0144; -.
DR   HOGENOM; CLU_036856_0_1_14; -.
DR   OMA; ISDHRVG; -.
DR   OrthoDB; 928964at2; -.
DR   PhylomeDB; P71496; -.
DR   Proteomes; UP000001928; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00093; Rel_fac_1; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004373; RF-1.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00019; prfA; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis.
FT   CHAIN           1..363
FT                   /note="Peptide chain release factor 1"
FT                   /id="PRO_0000177700"
FT   MOD_RES         237
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        309
FT                   /note="I -> F (in Ref. 1; BAA08795)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        355..363
FT                   /note="VAEQLKNNE -> SSWTIKKQWIKLFLIF (in Ref. 1)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   363 AA;  41157 MW;  A238B2784C07FBE1 CRC64;
     MNAKTYEALE TMQKRLFQIQ KDLEDENILK DIKKFTELNK EKANLEEVVE KFVEYKKAVD
     HIKDAKAILE NEKDSELIEL AKIELDENND KVEHLQQVIE EMLLPKDPND EKNVIIEIRG
     AAGGDEANIF AGDLLRMYKL YAETQNWKIN ILEASLGEAG GYSQVVFMIK GDRVYSKLKF
     ESGAHRVQRV PKTEAKGRIQ TSTATVAVLP EMSEVEIEIR SNDLRIDTYR ASGAGGQHVN
     TTDSAVRITH LPTGIVVTSQ DGRSQHDNKD IAMTMLRAKV YEAEVEKQQA QADATRKNAV
     GTGARSEKIR TYNYPQNRVT DHRVGLTLNK LDQVMEGNID EFIIALINEE QRQKVAEQLK
     NNE