RF1_MYCGE
ID RF1_MYCGE Reviewed; 359 AA.
AC P47500; Q49455;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Peptide chain release factor 1;
DE Short=RF-1;
GN Name=prfA; OrderedLocusNames=MG258;
OS Mycoplasma genitalium (strain ATCC 33530 / DSM 19775 / NCTC 10195 / G37)
OS (Mycoplasmoides genitalium).
OC Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX NCBI_TaxID=243273;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX PubMed=7569993; DOI=10.1126/science.270.5235.397;
RA Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A.,
RA Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M.,
RA Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L.,
RA Nguyen D.T., Utterback T.R., Saudek D.M., Phillips C.A., Merrick J.M.,
RA Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S.,
RA Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C.;
RT "The minimal gene complement of Mycoplasma genitalium.";
RL Science 270:397-403(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 241-353.
RC STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX PubMed=8253680; DOI=10.1128/jb.175.24.7918-7930.1993;
RA Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III;
RT "A survey of the Mycoplasma genitalium genome by using random sequencing.";
RL J. Bacteriol. 175:7918-7930(1993).
CC -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC translation in response to the peptide chain termination codons UAG and
CC UAA. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- PTM: Methylated by PrmC. Methylation increases the termination
CC efficiency of RF1 (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC family. {ECO:0000305}.
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DR EMBL; L43967; AAC71478.1; -; Genomic_DNA.
DR EMBL; U01800; AAD12326.1; -; Genomic_DNA.
DR PIR; E64228; E64228.
DR RefSeq; WP_009885793.1; NZ_AAGX01000005.1.
DR AlphaFoldDB; P47500; -.
DR SMR; P47500; -.
DR STRING; 243273.MG_258; -.
DR EnsemblBacteria; AAC71478; AAC71478; MG_258.
DR KEGG; mge:MG_258; -.
DR eggNOG; COG0216; Bacteria.
DR HOGENOM; CLU_036856_0_1_14; -.
DR OMA; ISDHRVG; -.
DR OrthoDB; 928964at2; -.
DR BioCyc; MGEN243273:G1GJ2-308-MON; -.
DR Proteomes; UP000000807; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00093; Rel_fac_1; 1.
DR InterPro; IPR005139; PCRF.
DR InterPro; IPR000352; Pep_chain_release_fac_I.
DR InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR InterPro; IPR004373; RF-1.
DR Pfam; PF03462; PCRF; 1.
DR Pfam; PF00472; RF-1; 1.
DR SMART; SM00937; PCRF; 1.
DR SUPFAM; SSF75620; SSF75620; 1.
DR TIGRFAMs; TIGR00019; prfA; 1.
DR PROSITE; PS00745; RF_PROK_I; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Methylation; Protein biosynthesis; Reference proteome.
FT CHAIN 1..359
FT /note="Peptide chain release factor 1"
FT /id="PRO_0000177702"
FT MOD_RES 236
FT /note="N5-methylglutamine"
FT /evidence="ECO:0000250"
FT CONFLICT 350..353
FT /note="KKQQ -> RTQL (in Ref. 2)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 359 AA; 40808 MW; 3DBF7D8E40E43A7C CRC64;
MDFDKQLFFN VEKIVELTEQ LEKDLNKPNL SFEQIKVINK ELKHKQPLIV KFKELQKLVE
NANEAEQILN NSSLKELHEE AKKELEKIKA SLPSLEEEIK FLLLPVDENN QKNVIVEIRP
AAGGDESCIF LSDLFNMYKN YCTSKNWTVE LNEIIPASVG INFVSFAVNG TDVFAKLKFE
SGVHRVQRVP LTEAKGRVHT STVTVAVLPQ LEEVEITINP SDLRIDTYRA SGAGGQHVNR
TESAVRITHL PTGIVVACQE GKSQFSNRDK AMKMLRAKLW ENAQNKQLST QADLRKSQVG
SGERAEKIRT YNYPQNRITD HRIKLTINKL NTVILGDLDE IIEALQADEK KQQLEKFIS
