ID   RF1_MYCLE               Reviewed;         361 AA.
AC   P45833;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   27-APR-2001, sequence version 2.
DT   25-MAY-2022, entry version 122.
DE   RecName: Full=Peptide chain release factor 1;
DE            Short=RF-1;
GN   Name=prfA; OrderedLocusNames=ML1134;
OS   Mycobacterium leprae (strain TN).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium.
OX   NCBI_TaxID=272631;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Smith D.R., Robison K.;
RL   Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TN;
RX   PubMed=11234002; DOI=10.1038/35059006;
RA   Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R.,
RA   Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E.,
RA   Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R.,
RA   Davies R.M., Devlin K., Duthoy S., Feltwell T., Fraser A., Hamlin N.,
RA   Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S.,
RA   Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M.,
RA   Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R.,
RA   Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R.,
RA   Barrell B.G.;
RT   "Massive gene decay in the leprosy bacillus.";
RL   Nature 409:1007-1011(2001).
CC   -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC       translation in response to the peptide chain termination codons UAG and
CC       UAA. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF1 (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA63095.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; U15186; AAA63095.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AL583920; CAC31515.1; -; Genomic_DNA.
DR   PIR; H87050; H87050.
DR   PIR; T09986; T09986.
DR   RefSeq; NP_301828.1; NC_002677.1.
DR   RefSeq; WP_010908152.1; NC_002677.1.
DR   AlphaFoldDB; P45833; -.
DR   SMR; P45833; -.
DR   STRING; 272631.ML1134; -.
DR   EnsemblBacteria; CAC31515; CAC31515; CAC31515.
DR   KEGG; mle:ML1134; -.
DR   PATRIC; fig|272631.5.peg.2056; -.
DR   Leproma; ML1134; -.
DR   eggNOG; COG0216; Bacteria.
DR   HOGENOM; CLU_036856_0_1_11; -.
DR   OMA; ISDHRVG; -.
DR   Proteomes; UP000000806; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00093; Rel_fac_1; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004373; RF-1.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00019; prfA; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..361
FT                   /note="Peptide chain release factor 1"
FT                   /id="PRO_0000177704"
FT   MOD_RES         240
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   361 AA;  39492 MW;  E8F7237CE1FBC235 CRC64;
     MAQPVQSIDV LLIEHAELEL ALADPELHSN PAEARKAGRR FARLAPIVAT HRKLISARDD
     LQTARELAAG DESFADEIAE LESRIAELTT QLTDMLAPHD PHGPDDIVLE VKSGEGGEES
     ALFAADLARM YIRYAERHGW TVTVLDETTS DLGGYKDATL AISHKGASDG DAVDGVWSRM
     KFEGGVHRVQ RVPVTESQGR VHTSAAGVLV YPEPEEIGEV HIDESDLRID VYRSSGKGGQ
     GVNTTDSAVR ITHLPTGVVV TCQNERSQLQ NKTRALQVLA ARLQAMAEEQ ALANASADRA
     SQIRTVDRSE RIRTYNFPEN RITDHRIGYK AHNLDQVLDG DLDALFDALS AADKQSRLQQ
     V