ID   RF1_MYCTO               Reviewed;         357 AA.
AC   P9WHG2; L0T686; P66016; Q10605;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 34.
DE   RecName: Full=Peptide chain release factor 1;
DE            Short=RF-1;
GN   Name=prfA; OrderedLocusNames=MT1338;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC       translation in response to the peptide chain termination codons UAG and
CC       UAA. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF1 (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000305}.
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DR   EMBL; AE000516; AAK45600.1; -; Genomic_DNA.
DR   PIR; G70773; G70773.
DR   RefSeq; WP_003406670.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WHG2; -.
DR   SMR; P9WHG2; -.
DR   EnsemblBacteria; AAK45600; AAK45600; MT1338.
DR   GeneID; 45425273; -.
DR   KEGG; mtc:MT1338; -.
DR   PATRIC; fig|83331.31.peg.1445; -.
DR   HOGENOM; CLU_036856_0_1_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00093; Rel_fac_1; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004373; RF-1.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00019; prfA; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis.
FT   CHAIN           1..357
FT                   /note="Peptide chain release factor 1"
FT                   /id="PRO_0000428192"
FT   MOD_RES         236
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   357 AA;  39036 MW;  F6DE97400EFC0663 CRC64;
     MTQPVQTIDV LLAEHAELEL ALADPALHSN PAEARRVGRR FARLAPIVAT HRKLTSARDD
     LETARELVAS DESFAAEVAA LEARVGELDA QLTDMLAPRD PHDADDIVLE VKSGEGGEES
     ALFAADLARM YIRYAERHGW AVTVLDETTS DLGGYKDATL AIASKADTPD GVWSRMKFEG
     GVHRVQRVPV TESQGRVHTS AAGVLVYPEP EEVGQVQIDE SDLRIDVFRS SGKGGQGVNT
     TDSAVRITHL PTGIVVTCQN ERSQLQNKTR ALQVLAARLQ AMAEEQALAD ASADRASQIR
     TVDRSERIRT YNFPENRITD HRIGYKSHNL DQVLDGDLDA LFDALSAADK QSRLRQS