ID   RF1_NOCFA               Reviewed;         356 AA.
AC   Q5Z0Z2;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=Peptide chain release factor 1 {ECO:0000255|HAMAP-Rule:MF_00093};
DE            Short=RF-1 {ECO:0000255|HAMAP-Rule:MF_00093};
GN   Name=prfA {ECO:0000255|HAMAP-Rule:MF_00093}; OrderedLocusNames=NFA_10540;
OS   Nocardia farcinica (strain IFM 10152).
OC   Bacteria; Actinobacteria; Corynebacteriales; Nocardiaceae; Nocardia.
OX   NCBI_TaxID=247156;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IFM 10152;
RX   PubMed=15466710; DOI=10.1073/pnas.0406410101;
RA   Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K.,
RA   Shiba T., Hattori M.;
RT   "The complete genomic sequence of Nocardia farcinica IFM 10152.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004).
CC   -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC       translation in response to the peptide chain termination codons UAG and
CC       UAA. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF1. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000255|HAMAP-Rule:MF_00093}.
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DR   EMBL; AP006618; BAD55899.1; -; Genomic_DNA.
DR   RefSeq; WP_011207584.1; NC_006361.1.
DR   AlphaFoldDB; Q5Z0Z2; -.
DR   SMR; Q5Z0Z2; -.
DR   STRING; 247156.NFA_10540; -.
DR   PRIDE; Q5Z0Z2; -.
DR   EnsemblBacteria; BAD55899; BAD55899; NFA_10540.
DR   GeneID; 61131876; -.
DR   KEGG; nfa:NFA_10540; -.
DR   eggNOG; COG0216; Bacteria.
DR   HOGENOM; CLU_036856_0_1_11; -.
DR   OMA; ISDHRVG; -.
DR   Proteomes; UP000006820; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00093; Rel_fac_1; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004373; RF-1.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00019; prfA; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..356
FT                   /note="Peptide chain release factor 1"
FT                   /id="PRO_0000177716"
FT   MOD_RES         235
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00093"
SQ   SEQUENCE   356 AA;  39024 MW;  8980B6EC6F8F61B7 CRC64;
     MTQPSAIDDI LAEHAGLETQ LADPTLHNDP SAARRVGKRF AELAPIMATY RKLESARADL
     TAARELAADD ASFAAEVPEL ERQVDELEQA LADLLAPRDP HDGDDVVLEV KSGEGGEESA
     LFAADLARMY MRYAERRGWR VEVLDATVSD LGGYKEATLS IKSRDAARDG VWSRFKFEGG
     VHRVQRVPVT ESQGRIHTSA AGVLIYPEPD EVAEVQIDES DLRIDVYRSS GKGGQGVNTT
     DSAVRITHLP TGIVVTCQNE RSQLQNKARA MQVLAARLQA LAEEQAEQEA AAGRASQIRT
     VDRSERIRTY NFPENRITDH RIGYKSHNLD AVLDGDLDAL LDALGKADRE ARMAAE