RF1_ORYSI
ID RF1_ORYSI Reviewed; 791 AA.
AC Q76C99; Q2PPE7;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 74.
DE RecName: Full=Protein Rf1, mitochondrial;
DE AltName: Full=Fertility restorer;
DE AltName: Full=Protein PPR;
DE AltName: Full=Restorer for CMS;
DE Flags: Precursor;
GN Name=Rf1; Synonyms=PPR791, PPR8-1, Rf-1, Rf-1A;
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND FUNCTION.
RC STRAIN=cv. BTR, and cv. Milyang 23; TISSUE=Anther;
RX PubMed=12782297; DOI=10.1016/s0014-5793(03)00480-0;
RA Kazama T., Toriyama K.;
RT "A pentatricopeptide repeat-containing gene that promotes the processing of
RT aberrant atp6 RNA of cytoplasmic male-sterile rice.";
RL FEBS Lett. 544:99-102(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=cv. MTC-10R;
RX PubMed=14968308; DOI=10.1007/s00122-004-1591-2;
RA Akagi H., Nakamura A., Yokozeki-Misono Y., Inagaki A., Takahashi H.,
RA Mori K., Fujimura T.;
RT "Positional cloning of the rice Rf-1 gene, a restorer of BT-type
RT cytoplasmic male sterility that encodes a mitochondria-targeting PPR
RT protein.";
RL Theor. Appl. Genet. 108:1449-1457(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. IR24; TISSUE=Leaf;
RX PubMed=14731253; DOI=10.1046/j.1365-313x.2003.01961.x;
RA Komori T., Ohta S., Murai N., Takakura Y., Kuraya Y., Suzuki S., Hiei Y.,
RA Imaseki H., Nitta N.;
RT "Map-based cloning of a fertility restorer gene, Rf-1, in rice (Oryza
RT sativa L.).";
RL Plant J. 37:315-325(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX PubMed=16489123; DOI=10.1105/tpc.105.038240;
RA Wang Z., Zou Y., Li X., Zhang Q., Chen L., Wu H., Su D., Chen Y., Guo J.,
RA Luo D., Long Y., Zhong Y., Liu Y.-G.;
RT "Cytoplasmic male sterility of rice with boro II cytoplasm is caused by a
RT cytotoxic peptide and is restored by two related PPR motif genes via
RT distinct modes of mRNA silencing.";
RL Plant Cell 18:676-687(2006).
CC -!- FUNCTION: Reduces the expression of the cytoplasmic male sterility
CC (CMS)-associated mitochondrial gene ORF79, encoding a cytotoxic
CC peptide. Can restore male fertility by blocking ORF79 production via
CC endonucleolytic cleavage of dicistronic ATP6/ORF79 mRNA. Promotes the
CC editing of ATP6 mRNAs independently of its cleavage function.
CC {ECO:0000269|PubMed:12782297, ECO:0000269|PubMed:16489123}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB110016; BAC77666.2; -; mRNA.
DR EMBL; AB106867; BAC77665.2; -; Genomic_DNA.
DR EMBL; AB112808; BAD13708.1; -; mRNA.
DR EMBL; AB179840; BAD20283.1; -; Genomic_DNA.
DR EMBL; AB110443; BAD08214.1; -; Genomic_DNA.
DR EMBL; DQ311053; ABC42330.1; -; Genomic_DNA.
DR AlphaFoldDB; Q76C99; -.
DR SMR; Q76C99; -.
DR PRIDE; Q76C99; -.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR Gene3D; 1.25.40.10; -; 8.
DR InterPro; IPR002885; Pentatricopeptide_repeat.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR Pfam; PF01535; PPR; 1.
DR Pfam; PF12854; PPR_1; 4.
DR Pfam; PF13041; PPR_2; 4.
DR Pfam; PF13812; PPR_3; 1.
DR SUPFAM; SSF48452; SSF48452; 1.
DR TIGRFAMs; TIGR00756; PPR; 16.
DR PROSITE; PS51375; PPR; 19.
PE 2: Evidence at transcript level;
KW Mitochondrion; Repeat; Transit peptide.
FT TRANSIT 1..27
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 28..791
FT /note="Protein Rf1, mitochondrial"
FT /id="PRO_0000022223"
FT REPEAT 86..120
FT /note="PPR 1"
FT REPEAT 121..156
FT /note="PPR 2"
FT REPEAT 157..194
FT /note="PPR 3"
FT REPEAT 195..229
FT /note="PPR 4"
FT REPEAT 230..264
FT /note="PPR 5"
FT REPEAT 265..299
FT /note="PPR 6"
FT REPEAT 300..334
FT /note="PPR 7"
FT REPEAT 335..369
FT /note="PPR 8"
FT REPEAT 370..404
FT /note="PPR 9"
FT REPEAT 405..439
FT /note="PPR 10"
FT REPEAT 440..474
FT /note="PPR 11"
FT REPEAT 475..509
FT /note="PPR 12"
FT REPEAT 510..544
FT /note="PPR 13"
FT REPEAT 545..579
FT /note="PPR 14"
FT REPEAT 580..614
FT /note="PPR 15"
FT REPEAT 615..649
FT /note="PPR 16"
FT REPEAT 650..684
FT /note="PPR 17"
FT REPEAT 685..719
FT /note="PPR 18"
FT REPEAT 720..754
FT /note="PPR 19"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 383
FT /note="K -> N (in Ref. 4; ABC42330)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 791 AA; 87614 MW; D2B7DA3CD31EE74E CRC64;
MARRAASRAV GALRSDGSIQ GRGGRAGGSG AEDARHVFDE LLRRGRGASI YGLNRALADV
ARDSPAAAVS RYNRMARAGA DEVTPDLCTY GILIGCCCRA GRLDLGFAAL GNVIKKGFRV
DAIAFTPLLK GLCADKRTSD AMDIVLRRMT ELGCIPNVFS YNILLKGLCD ENRSQEALEL
LHMMADDRGG GSPPDVVSYT TVINGFFKEG DSDKAYSTYH EMLDRGILPD VVTYNSIIAA
LCKAQAMDKA MEVLNTMVKN GVMPDCMTYN SILHGYCSSG QPKEAIGFLK KMRSDGVEPD
VVTYSLLMDY LCKNGRCMEA RKIFDSMTKR GLKPEITTYG TLLQGYATKG ALVEMHGLLD
LMVRNGIHPD HYVFSILICA YAKQGKVDQA MLVFSKMRQQ GLNPNAVTYG AVIGILCKSG
RVEDAMLYFE QMIDEGLSPG NIVYNSLIHG LCTCNKWERA EELILEMLDR GICLNTIFFN
SIIDSHCKEG RVIESEKLFE LMVRIGVKPN VITYNTLING YCLAGKMDEA MKLLSGMVSV
GLKPNTVTYS TLINGYCKIS RMEDALVLFK EMESSGVSPD IITYNIILQG LFQTRRTAAA
KELYVRITES GTQIELSTYN IILHGLCKNK LTDDALQMFQ NLCLMDLKLE ARTFNIMIDA
LLKVGRNDEA KDLFVAFSSN GLVPNYWTYR LMAENIIGQG LLEELDQLFL SMEDNGCTVD
SGMLNFIVRE LLQRGEITRA GTYLSMIDEK HFSLEASTAS LFIDLLSGGK YQEYYRFLPE
KYKSFIESLS C
