ID   RF1_PHYAS               Reviewed;         354 AA.
AC   B1V950;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 1.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=Peptide chain release factor 1 {ECO:0000255|HAMAP-Rule:MF_00093};
DE            Short=RF-1 {ECO:0000255|HAMAP-Rule:MF_00093};
GN   Name=prfA {ECO:0000255|HAMAP-Rule:MF_00093}; OrderedLocusNames=PA0147;
OS   Phytoplasma australiense.
OC   Bacteria; Tenericutes; Mollicutes; Acholeplasmatales; Acholeplasmataceae;
OC   Candidatus Phytoplasma; 16SrXII (Stolbur group).
OX   NCBI_TaxID=59748;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=18359806; DOI=10.1128/jb.01301-07;
RA   Tran-Nguyen L.T., Kube M., Schneider B., Reinhardt R., Gibb K.S.;
RT   "Comparative genome analysis of 'Candidatus Phytoplasma australiense'
RT   (subgroup tuf-Australia I; rp-A) and 'Ca. Phytoplasma asteris' strains OY-M
RT   and AY-WB.";
RL   J. Bacteriol. 190:3979-3991(2008).
CC   -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC       translation in response to the peptide chain termination codons UAG and
CC       UAA. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF1. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000255|HAMAP-Rule:MF_00093}.
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DR   EMBL; AM422018; CAM11482.1; -; Genomic_DNA.
DR   RefSeq; WP_012358747.1; NC_010544.1.
DR   AlphaFoldDB; B1V950; -.
DR   SMR; B1V950; -.
DR   STRING; 59748.PA0147; -.
DR   EnsemblBacteria; CAM11482; CAM11482; PA0147.
DR   KEGG; pal:PA0147; -.
DR   eggNOG; COG0216; Bacteria.
DR   OMA; ISDHRVG; -.
DR   Proteomes; UP000008323; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00093; Rel_fac_1; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004373; RF-1.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00019; prfA; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..354
FT                   /note="Peptide chain release factor 1"
FT                   /id="PRO_1000117251"
FT   MOD_RES         232
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00093"
SQ   SEQUENCE   354 AA;  40358 MW;  875396565B368818 CRC64;
     MLARLKKIQQ KYQNLQQDLL QNQSISNKIN IFKNLSKLEP IVELFQQYLT LEEQLTNITK
     ALNQDQIEDK EILELAQKEK ETLSQQKKVL WNQLRILLLP QDPEDQKNVI LEIKGAVGGN
     EANLFAADLL RTYMKYAESK KWKVEILNLH PSIKGGLSSV ELLISGQNIY SFLKYESGVH
     RVQRVPATEV QGRIHTSTAI VLVLPEAKEV EIKIDWNDIR TDTFNSSGPG GQSVNTTKSA
     VRLSHLPSGI SVACQEGKSQ HENKEKAFTL LKARIYNQIL NAKQEAENKH RKSLVGTGDR
     SEKIRTYNYP QNRITDHRIG LTLQKLDIIM EGKLDFIIEP LIDATQKEKL VQSE