ID   RF1_POLNA               Reviewed;         373 AA.
AC   A1VKB6;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Peptide chain release factor 1 {ECO:0000255|HAMAP-Rule:MF_00093};
DE            Short=RF-1 {ECO:0000255|HAMAP-Rule:MF_00093};
GN   Name=prfA {ECO:0000255|HAMAP-Rule:MF_00093}; OrderedLocusNames=Pnap_0775;
OS   Polaromonas naphthalenivorans (strain CJ2).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Polaromonas.
OX   NCBI_TaxID=365044;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CJ2;
RX   PubMed=19453698; DOI=10.1111/j.1462-2920.2009.01947.x;
RA   Yagi J.M., Sims D., Brettin T., Bruce D., Madsen E.L.;
RT   "The genome of Polaromonas naphthalenivorans strain CJ2, isolated from coal
RT   tar-contaminated sediment, reveals physiological and metabolic versatility
RT   and evolution through extensive horizontal gene transfer.";
RL   Environ. Microbiol. 11:2253-2270(2009).
CC   -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC       translation in response to the peptide chain termination codons UAG and
CC       UAA. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF1. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000255|HAMAP-Rule:MF_00093}.
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DR   EMBL; CP000529; ABM36094.1; -; Genomic_DNA.
DR   RefSeq; WP_011800189.1; NC_008781.1.
DR   AlphaFoldDB; A1VKB6; -.
DR   SMR; A1VKB6; -.
DR   STRING; 365044.Pnap_0775; -.
DR   EnsemblBacteria; ABM36094; ABM36094; Pnap_0775.
DR   KEGG; pna:Pnap_0775; -.
DR   eggNOG; COG0216; Bacteria.
DR   HOGENOM; CLU_036856_0_1_4; -.
DR   OMA; ISDHRVG; -.
DR   OrthoDB; 928964at2; -.
DR   Proteomes; UP000000644; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00093; Rel_fac_1; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004373; RF-1.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00019; prfA; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..373
FT                   /note="Peptide chain release factor 1"
FT                   /id="PRO_1000004929"
FT   MOD_RES         245
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00093"
SQ   SEQUENCE   373 AA;  41062 MW;  184AA02245144C67 CRC64;
     MKPFLRHTLD RQLLRLHELD ALLSASDVVS DLDRFRALTR EHAEASVVAE QYNRLTAREA
     DLASAESMLA EAKNDPDMAE MAEEEINAAK TDIAQLDEAL QLMLIPKDPD DRRAAFIEIR
     AGTGGDESAL FAGDLFRLYT RFAERKRWQV EIISESPSEL GGYKEVVLRI DGPTDAMGVG
     VYGKLRFESG GHRVQRVPAT ETQGRIHTSA CTVAVLPEPD ETVAIQINPA DLRIDTYRAS
     GAGGQHINKT DSAVRITHLP TGIVAECQEG RSQHGNKAQA MKVLTARIHE KERSAQAAKD
     AAMRKGLIGS GDRSDRIRTY NFPQGRLTDH RINLTLYKLL TIMEGDLDDV VTALQVARGA
     EQLNELETAN NGG