RF1_PSEAE
ID RF1_PSEAE Reviewed; 360 AA.
AC P42806; Q9HVC7;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2001, sequence version 2.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Peptide chain release factor 1;
DE Short=RF-1;
GN Name=prfA; Synonyms=prf1; OrderedLocusNames=PA4665;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-96.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=7883699; DOI=10.1128/jb.177.6.1435-1443.1995;
RA Hungerer C., Troup B., Roemling U., Jahn D.;
RT "Regulation of the hemA gene during 5-aminolevulinic acid formation in
RT Pseudomonas aeruginosa.";
RL J. Bacteriol. 177:1435-1443(1995).
CC -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC translation in response to the peptide chain termination codons UAG and
CC UAA. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- PTM: Methylated by PrmC. Methylation increases the termination
CC efficiency of RF1 (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC family. {ECO:0000305}.
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DR EMBL; AE004091; AAG08052.1; -; Genomic_DNA.
DR EMBL; X82071; CAA57573.1; -; Genomic_DNA.
DR PIR; B83063; B83063.
DR PIR; S49377; S49377.
DR RefSeq; NP_253354.1; NC_002516.2.
DR RefSeq; WP_003114692.1; NZ_QZGE01000029.1.
DR AlphaFoldDB; P42806; -.
DR SMR; P42806; -.
DR STRING; 287.DR97_1974; -.
DR PaxDb; P42806; -.
DR PRIDE; P42806; -.
DR EnsemblBacteria; AAG08052; AAG08052; PA4665.
DR GeneID; 881360; -.
DR KEGG; pae:PA4665; -.
DR PATRIC; fig|208964.12.peg.4887; -.
DR PseudoCAP; PA4665; -.
DR HOGENOM; CLU_036856_0_1_6; -.
DR InParanoid; P42806; -.
DR OMA; ISDHRVG; -.
DR PhylomeDB; P42806; -.
DR BioCyc; PAER208964:G1FZ6-4761-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00093; Rel_fac_1; 1.
DR InterPro; IPR005139; PCRF.
DR InterPro; IPR000352; Pep_chain_release_fac_I.
DR InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR InterPro; IPR004373; RF-1.
DR Pfam; PF03462; PCRF; 1.
DR Pfam; PF00472; RF-1; 1.
DR SMART; SM00937; PCRF; 1.
DR SUPFAM; SSF75620; SSF75620; 1.
DR TIGRFAMs; TIGR00019; prfA; 1.
DR PROSITE; PS00745; RF_PROK_I; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Methylation; Protein biosynthesis; Reference proteome.
FT CHAIN 1..360
FT /note="Peptide chain release factor 1"
FT /id="PRO_0000177723"
FT MOD_RES 237
FT /note="N5-methylglutamine"
FT /evidence="ECO:0000250"
FT CONFLICT 33..34
FT /note="TR -> DP (in Ref. 2; CAA57573)"
FT /evidence="ECO:0000305"
FT CONFLICT 51
FT /note="A -> R (in Ref. 2; CAA57573)"
FT /evidence="ECO:0000305"
FT CONFLICT 93..96
FT /note="ALGD -> PRRQ (in Ref. 2; CAA57573)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 360 AA; 40041 MW; 572FB2006CC8626E CRC64;
MKASLLKKLD VLSDRYEELT ALLGDAEVIS DQTRFRAYSR EYAEVEPVIL AFRDYRKVQA
DLEGAQALLK DSDPELRDLA EEEVAEARGR LAALGDSLQR MLLPKDPNDS RNVFLEIRAG
TGGDEAAIFS GDLFRMYSRY AERQGWRIET LSENEGEHGG YKEVIARVEG DNVYAKLKFE
SGAHRVQRVP ETESQGRIHT SACTVAVLPE PDEQAAIEIN PADLRVDTYR SSGAGGQHVN
KTDSAVRITH IPSGIVVECQ EERSQHKNRA KAMAWLAAKL NDQQQAAAQQ AIASTRKLLV
GSGDRSERIR TYNFPQGRVT DHRINLTLYS LGEVMEGAVE QVIEPLLQEY QADQLAALGD
