ID   RF1_PSYWF               Reviewed;         364 AA.
AC   A5WFG1;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Peptide chain release factor 1 {ECO:0000255|HAMAP-Rule:MF_00093};
DE            Short=RF-1 {ECO:0000255|HAMAP-Rule:MF_00093};
GN   Name=prfA {ECO:0000255|HAMAP-Rule:MF_00093};
GN   OrderedLocusNames=PsycPRwf_1457;
OS   Psychrobacter sp. (strain PRwf-1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Moraxellales; Moraxellaceae;
OC   Psychrobacter.
OX   NCBI_TaxID=349106;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PRwf-1;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Kim E., Tiedje J., Richardson P.;
RT   "Complete sequence of chromosome of Psychrobacter sp. PRwf-1.";
RL   Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC       translation in response to the peptide chain termination codons UAG and
CC       UAA. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF1. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000255|HAMAP-Rule:MF_00093}.
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DR   EMBL; CP000713; ABQ94402.1; -; Genomic_DNA.
DR   RefSeq; WP_011960698.1; NC_009524.1.
DR   AlphaFoldDB; A5WFG1; -.
DR   SMR; A5WFG1; -.
DR   STRING; 349106.PsycPRwf_1457; -.
DR   EnsemblBacteria; ABQ94402; ABQ94402; PsycPRwf_1457.
DR   KEGG; prw:PsycPRwf_1457; -.
DR   eggNOG; COG0216; Bacteria.
DR   HOGENOM; CLU_036856_0_1_6; -.
DR   OMA; ISDHRVG; -.
DR   OrthoDB; 928964at2; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00093; Rel_fac_1; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004373; RF-1.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00019; prfA; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis.
FT   CHAIN           1..364
FT                   /note="Peptide chain release factor 1"
FT                   /id="PRO_1000071265"
FT   MOD_RES         238
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00093"
SQ   SEQUENCE   364 AA;  40987 MW;  6CBC85D3DD7398D2 CRC64;
     MKDSLRERLD QMVDRFEEVT ALLSDPDTIS DNNKFRELSM EHSELSEITA LWQRYRQAEI
     DKKDAQEMIN DASDPDMKEM MQEEIDSASR DIEAMEEELN VMMLPKDPND KVPAFLEIRA
     GTGGDEAAIF SGDLFRMYER YASSQGWTIE VLSANEGEHG GYKEIISRVS GNDVYGRLKF
     ESGAHRVQRV PETESQGRVH TSACTVAVMP EVEIDDTVEL NPADIRMDTF RSSGAGGQHV
     NTTDSAVRLT HIPTGVVAEC QQERSQHKNR AKAMQMLIAR IQQAKVQEQV DTADALRRNL
     VGSGDRSERI RTYNFPQGRM TDHRINLTLY KLDAIMEGDL TELLDALNRE HQADLMASIG
     GGDD