ID   RF1_RIPO1               Reviewed;         370 AA.
AC   B7K205;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Peptide chain release factor 1 {ECO:0000255|HAMAP-Rule:MF_00093};
DE            Short=RF-1 {ECO:0000255|HAMAP-Rule:MF_00093};
GN   Name=prfA {ECO:0000255|HAMAP-Rule:MF_00093};
GN   OrderedLocusNames=PCC8801_0208;
OS   Rippkaea orientalis (strain PCC 8801) (Cyanothece sp. (strain PCC 8801)).
OC   Bacteria; Cyanobacteria; Oscillatoriophycideae; Chroococcales;
OC   Aphanothecaceae; Rippkaea; Rippkaea orientalis.
OX   NCBI_TaxID=41431;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 8801;
RX   PubMed=21972240; DOI=10.1128/mbio.00214-11;
RA   Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H.,
RA   Sherman L.A., Pakrasi H.B.;
RT   "Novel metabolic attributes of the genus Cyanothece, comprising a group of
RT   unicellular nitrogen-fixing Cyanobacteria.";
RL   MBio 2:E214-E214(2011).
CC   -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC       translation in response to the peptide chain termination codons UAG and
CC       UAA. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF1. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000255|HAMAP-Rule:MF_00093}.
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DR   EMBL; CP001287; ACK64312.1; -; Genomic_DNA.
DR   RefSeq; WP_012593589.1; NC_011726.1.
DR   AlphaFoldDB; B7K205; -.
DR   SMR; B7K205; -.
DR   STRING; 41431.PCC8801_0208; -.
DR   EnsemblBacteria; ACK64312; ACK64312; PCC8801_0208.
DR   KEGG; cyp:PCC8801_0208; -.
DR   eggNOG; COG0216; Bacteria.
DR   HOGENOM; CLU_036856_0_1_3; -.
DR   OMA; ISDHRVG; -.
DR   OrthoDB; 928964at2; -.
DR   Proteomes; UP000008204; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00093; Rel_fac_1; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004373; RF-1.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00019; prfA; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..370
FT                   /note="Peptide chain release factor 1"
FT                   /id="PRO_1000117233"
FT   MOD_RES         239
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00093"
SQ   SEQUENCE   370 AA;  41563 MW;  70C4C52CCE2E89C8 CRC64;
     MAESYLLEKL QSVDQTYQEL THRLADPDIA TNPDELQRVA RLRSSLEETV LTYDSWKQTQ
     EELIGAKQIL KDAGGDPEMR EMAALEVEEL AEKLEELEKK LKILLLPRDP NDDKNIMLEI
     RAGTGGDEAS IWAGDLVRMY SRYAESQKWT VKLVSESLAD MGGFKEAILE IQGDRVYSKL
     KFEAGVHRVQ RVPVTEAGGR VHTSTATVAI MPEVDDVEVQ IDAKDIEITT ARSGGAGGQN
     VNKVETAVDL FYKPLGIRIF CTEERSQLQN RERAMQILRA KLYDLKLREQ QDAVSSMRKS
     QVGTGARSEK IRTYNYKDNR VTDHRLGQNF SLAGTLEGDI EEIIQSCISQ DQQERLEELA
     ASPETASAIN