ID   RF1_STAA8               Reviewed;         358 AA.
AC   Q2FWE0;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=Peptide chain release factor 1 {ECO:0000255|HAMAP-Rule:MF_00093};
DE            Short=RF-1 {ECO:0000255|HAMAP-Rule:MF_00093};
GN   Name=prfA {ECO:0000255|HAMAP-Rule:MF_00093};
GN   OrderedLocusNames=SAOUHSC_02359;
OS   Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 8325 / PS 47;
RA   Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT   "The Staphylococcus aureus NCTC 8325 genome.";
RL   (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL   Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL   D.C. (2006).
CC   -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC       translation in response to the peptide chain termination codons UAG and
CC       UAA. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF1. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000255|HAMAP-Rule:MF_00093}.
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DR   EMBL; CP000253; ABD31390.1; -; Genomic_DNA.
DR   RefSeq; WP_000460242.1; NZ_LS483365.1.
DR   RefSeq; YP_500835.1; NC_007795.1.
DR   AlphaFoldDB; Q2FWE0; -.
DR   SMR; Q2FWE0; -.
DR   STRING; 1280.SAXN108_2363; -.
DR   EnsemblBacteria; ABD31390; ABD31390; SAOUHSC_02359.
DR   GeneID; 3919402; -.
DR   KEGG; sao:SAOUHSC_02359; -.
DR   PATRIC; fig|93061.5.peg.2136; -.
DR   eggNOG; COG0216; Bacteria.
DR   HOGENOM; CLU_036856_0_1_9; -.
DR   OMA; ISDHRVG; -.
DR   PRO; PR:Q2FWE0; -.
DR   Proteomes; UP000008816; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00093; Rel_fac_1; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004373; RF-1.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00019; prfA; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..358
FT                   /note="Peptide chain release factor 1"
FT                   /id="PRO_0000263359"
FT   MOD_RES         233
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00093"
SQ   SEQUENCE   358 AA;  40350 MW;  CA3D92818704954D CRC64;
     MFDQLDIVEE RYEQLNELLS DPDVVNDSDK LRKYSKEQAD LQKTVDVYRN YKAKKEELAD
     IEEMLSETDD KEEVEMLKEE SNGIKAELPN LEEELKILLI PKDPNDDKDV IVEIRAAAGG
     DEAAIFAGDL MRMYSKYAES QGFKTEIVEA SESDHGGYKE ISFSVSGNGA YSKLKFENGA
     HRVQRVPETE SGGRIHTSTA TVAVLPEVED VEIEIRNEDL KIDTYRSSGA GGQHVNTTDS
     AVRITHLPTG VIATSSEKSQ IQNREKAMKV LKARLYDMKV QEEQQKYASQ RKSAVGTGDR
     SERIRTYNYP QSRVTDHRIG LTLQKLGQIM EGHLEEIIDA LTLSEQTDKL KELNNGEL