ID   RF1_SYNY3               Reviewed;         365 AA.
AC   P74707;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   25-MAY-2022, entry version 132.
DE   RecName: Full=Peptide chain release factor 1;
DE            Short=RF-1;
GN   Name=prfA; OrderedLocusNames=sll1110;
OS   Synechocystis sp. (strain PCC 6803 / Kazusa).
OC   Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC   unclassified Synechocystis.
OX   NCBI_TaxID=1111708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA   Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA   Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA   Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA   Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence analysis of the genome of the unicellular cyanobacterium
RT   Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT   genome and assignment of potential protein-coding regions.";
RL   DNA Res. 3:109-136(1996).
CC   -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC       translation in response to the peptide chain termination codons UAG and
CC       UAA. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF1 (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000305}.
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DR   EMBL; BA000022; BAA18826.1; -; Genomic_DNA.
DR   PIR; S76914; S76914.
DR   AlphaFoldDB; P74707; -.
DR   SMR; P74707; -.
DR   IntAct; P74707; 2.
DR   STRING; 1148.1653916; -.
DR   PaxDb; P74707; -.
DR   EnsemblBacteria; BAA18826; BAA18826; BAA18826.
DR   KEGG; syn:sll1110; -.
DR   eggNOG; COG0216; Bacteria.
DR   InParanoid; P74707; -.
DR   OMA; ISDHRVG; -.
DR   PhylomeDB; P74707; -.
DR   Proteomes; UP000001425; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   GO; GO:0009658; P:chloroplast organization; IBA:GO_Central.
DR   GO; GO:0010027; P:thylakoid membrane organization; IBA:GO_Central.
DR   GO; GO:0006415; P:translational termination; IBA:GO_Central.
DR   HAMAP; MF_00093; Rel_fac_1; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004373; RF-1.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00019; prfA; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..365
FT                   /note="Peptide chain release factor 1"
FT                   /id="PRO_0000177760"
FT   MOD_RES         239
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   365 AA;  41126 MW;  D565402A35E27EAC CRC64;
     MAETYLLDKL ASVEQTYQEL TRMLADPDIA TNPDELQRVA KARSSLEETV DTYETWKRSQ
     EDLKGARQIV KESGNDPEMR EMAQLEVEEL ENAIGELEKR LTILLLPKDP NDEKNIMLEI
     RAGTGGDEAS IWAGDLVRMY TRYAESQGWK VSLLSESLAD MGGFKEAILE VKGDRVYSQL
     KFEAGVHRVQ RVPVTEAGGR VHTSTATVAI MPEVDDVEVK IDPKDIEMST ARSGGAGGQN
     VNKVETAVDL FHKPTGIRIF CTEERSQLQN RERAMQILRA KLYDMMLQEQ NDAISSNRRS
     QVGTGSRSEK IRTYNYKDNR VTDHRLGRNF DLNTALEGEI HTIIESCISQ DQQERLAELA
     EATNN