ID   RF1_TRIV2               Reviewed;         366 AA.
AC   Q3MF91;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2005, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Peptide chain release factor 1 {ECO:0000255|HAMAP-Rule:MF_00093};
DE            Short=RF-1 {ECO:0000255|HAMAP-Rule:MF_00093};
GN   Name=prfA {ECO:0000255|HAMAP-Rule:MF_00093}; OrderedLocusNames=Ava_0721;
OS   Trichormus variabilis (strain ATCC 29413 / PCC 7937) (Anabaena variabilis).
OC   Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Trichormus.
OX   NCBI_TaxID=240292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29413 / PCC 7937;
RX   PubMed=25197444; DOI=10.4056/sigs.3899418;
RA   Thiel T., Pratte B.S., Zhong J., Goodwin L., Copeland A., Lucas S., Han C.,
RA   Pitluck S., Land M.L., Kyrpides N.C., Woyke T.;
RT   "Complete genome sequence of Anabaena variabilis ATCC 29413.";
RL   Stand. Genomic Sci. 9:562-573(2014).
CC   -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC       translation in response to the peptide chain termination codons UAG and
CC       UAA. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF1. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000255|HAMAP-Rule:MF_00093}.
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DR   EMBL; CP000117; ABA20345.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q3MF91; -.
DR   SMR; Q3MF91; -.
DR   STRING; 240292.Ava_0721; -.
DR   PRIDE; Q3MF91; -.
DR   EnsemblBacteria; ABA20345; ABA20345; Ava_0721.
DR   KEGG; ava:Ava_0721; -.
DR   eggNOG; COG0216; Bacteria.
DR   HOGENOM; CLU_036856_0_1_3; -.
DR   OMA; ISDHRVG; -.
DR   Proteomes; UP000002533; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00093; Rel_fac_1; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004373; RF-1.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00019; prfA; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis.
FT   CHAIN           1..366
FT                   /note="Peptide chain release factor 1"
FT                   /id="PRO_0000263229"
FT   MOD_RES         239
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00093"
SQ   SEQUENCE   366 AA;  41233 MW;  7F01CA49CED867D5 CRC64;
     MAESYLLEKL KSVEQTFNEL TRRLADPDTA RNPDEYQKIA KSRSSLEEVV NTYETWKIAQ
     EELIGARQVL KESNSDPELQ EIAALEVNEL EEKIKYLETR LKVLLLPRDP NDDKNIMLEI
     RAGTGGDEAS IWAGDLLRMY SRYADTQGWR VKLVSESLGE MGGFKEVILE IQGDSVYSKL
     KFEAGVHRVQ RVPATEAGGR VHTSTATVAI MPEVDEVEIH IDPKDIEMTT ARSGGAGGQN
     VNKVETAVDL MHKPTGIRIF CTEERSQLQN KERAMQILRA KLYEIKLREQ QEEVTSMRRS
     QVGTGSRSEK IRTYNYKDSR ATDHRLGQNF SLNPVLEGDL ETVIQSCISQ DQQERLAELA
     TSSAAG