RF1_VIBC3
ID RF1_VIBC3 Reviewed; 362 AA.
AC A5F696; C3M3D6;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 12-JUN-2007, sequence version 1.
DT 25-MAY-2022, entry version 85.
DE RecName: Full=Peptide chain release factor 1 {ECO:0000255|HAMAP-Rule:MF_00093};
DE Short=RF-1 {ECO:0000255|HAMAP-Rule:MF_00093};
GN Name=prfA {ECO:0000255|HAMAP-Rule:MF_00093};
GN OrderedLocusNames=VC0395_A1756, VC395_2293;
OS Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 /
OS O395).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=345073;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RA Heidelberg J.;
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX PubMed=19115014; DOI=10.1371/journal.pone.0004053;
RA Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J.,
RA Wang W., Wang J., Qian W., Li D., Wang L.;
RT "A recalibrated molecular clock and independent origins for the cholera
RT pandemic clones.";
RL PLoS ONE 3:E4053-E4053(2008).
CC -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC translation in response to the peptide chain termination codons UAG and
CC UAA. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00093}.
CC -!- PTM: Methylated by PrmC. Methylation increases the termination
CC efficiency of RF1. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC family. {ECO:0000255|HAMAP-Rule:MF_00093}.
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DR EMBL; CP000627; ABQ20878.1; -; Genomic_DNA.
DR EMBL; CP001235; ACP10285.1; -; Genomic_DNA.
DR RefSeq; WP_000647701.1; NZ_JAACZH010000001.1.
DR AlphaFoldDB; A5F696; -.
DR SMR; A5F696; -.
DR STRING; 345073.VC395_2293; -.
DR EnsemblBacteria; ABQ20878; ABQ20878; VC0395_A1756.
DR GeneID; 57740791; -.
DR GeneID; 66939982; -.
DR KEGG; vco:VC0395_A1756; -.
DR KEGG; vcr:VC395_2293; -.
DR PATRIC; fig|345073.21.peg.2210; -.
DR eggNOG; COG0216; Bacteria.
DR HOGENOM; CLU_036856_0_1_6; -.
DR OMA; ISDHRVG; -.
DR Proteomes; UP000000249; Chromosome 2.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00093; Rel_fac_1; 1.
DR InterPro; IPR005139; PCRF.
DR InterPro; IPR000352; Pep_chain_release_fac_I.
DR InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR InterPro; IPR004373; RF-1.
DR Pfam; PF03462; PCRF; 1.
DR Pfam; PF00472; RF-1; 1.
DR SMART; SM00937; PCRF; 1.
DR SUPFAM; SSF75620; SSF75620; 1.
DR TIGRFAMs; TIGR00019; prfA; 1.
DR PROSITE; PS00745; RF_PROK_I; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Methylation; Protein biosynthesis.
FT CHAIN 1..362
FT /note="Peptide chain release factor 1"
FT /id="PRO_1000071267"
FT REGION 289..308
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 289..304
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 237
FT /note="N5-methylglutamine"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00093"
SQ SEQUENCE 362 AA; 40221 MW; A564DDBDBD362223 CRC64;
MKASILSKLE SLVERYEEVQ HLLGDPTVIG DQNKFRALSK EYSQLEEITQ CFQAYQQAKE
DLVAAEEMAQ EDDAEMREMA QDEIKAAKAA IERLTDELQI LLLPKDPNDD RNCFLEIRAG
AGGDEAGIFA GDLFRMYSRF AEKKGWRIEV MSSSEAEHGG YKEMIAKVNG DGAYGTLKFE
SGGHRVQRVP ATEAQGRIHT SACTVAVMPE IPEAEIPEIK ASDLKIDTFR SSGAGGQHVN
TTDSAIRITH LPTGIVVECQ DERSQHKNKA KAMSVLAARI AQAEESKRAA EISDTRRNLL
GSGDRSDRIR TYNYPQGRVS DHRINLTVYR LTEVMEGDMQ SLIDPVIHEH QADQLAALAD
QN
