ID   RF1_WIGBR               Reviewed;         364 AA.
AC   Q8D2K9;
DT   23-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Peptide chain release factor 1 {ECO:0000255|HAMAP-Rule:MF_00093};
DE            Short=RF-1 {ECO:0000255|HAMAP-Rule:MF_00093};
GN   Name=prfA {ECO:0000255|HAMAP-Rule:MF_00093}; OrderedLocusNames=WIGBR3450;
OS   Wigglesworthia glossinidia brevipalpis.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Wigglesworthia.
OX   NCBI_TaxID=36870;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12219091; DOI=10.1038/ng986;
RA   Akman L., Yamashita A., Watanabe H., Oshima K., Shiba T., Hattori M.,
RA   Aksoy S.;
RT   "Genome sequence of the endocellular obligate symbiont of tsetse flies,
RT   Wigglesworthia glossinidia.";
RL   Nat. Genet. 32:402-407(2002).
CC   -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC       translation in response to the peptide chain termination codons UAG and
CC       UAA. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF1. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000255|HAMAP-Rule:MF_00093}.
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DR   EMBL; BA000021; BAC24491.1; -; Genomic_DNA.
DR   RefSeq; WP_011070149.1; NC_004344.2.
DR   AlphaFoldDB; Q8D2K9; -.
DR   SMR; Q8D2K9; -.
DR   STRING; 36870.25166301; -.
DR   PRIDE; Q8D2K9; -.
DR   EnsemblBacteria; BAC24491; BAC24491; BAC24491.
DR   KEGG; wbr:prfA; -.
DR   eggNOG; COG0216; Bacteria.
DR   HOGENOM; CLU_036856_0_1_6; -.
DR   OMA; ISDHRVG; -.
DR   OrthoDB; 928964at2; -.
DR   Proteomes; UP000000562; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00093; Rel_fac_1; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004373; RF-1.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00019; prfA; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..364
FT                   /note="Peptide chain release factor 1"
FT                   /id="PRO_0000177770"
FT   MOD_RES         241
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00093"
SQ   SEQUENCE   364 AA;  42417 MW;  9BCBB8BFD35BDAC0 CRC64;
     MKFSFEKNYA LVQKLKKLKN RYKEIKIILS DPFSAKKQKN FFLLSKEYSK LNILNKYLKS
     LESINNTIES TKTMLLDPEM HDLAKNEINI YKNKKKLIEK KIILYLIPNE DEYICNCFIE
     IRSGTGGKEA SLFVRDLCRM YIKYSEIKNW KTDIINYSNS EHGGYKEVII KICNKNAYNK
     LKFESGGHRV QRIPETESQG RIHTSTCTIA VMPEMLDFKL PKIKTSDLRI DTFRSSGAGG
     QHINTTDSAI RITHIPTNIV VECQDERSQH KNKSKAMLVL KSRLQANLLK NKKQKEEIIR
     KSLLGSGDRS DRIRTYNFPQ GRITDHRINL TIYKIDEVMD GNLNILINPI LEKYELEKIK
     EILK