ID   RF2F_ORYSJ              Reviewed;         152 AA.
AC   F1SZ42;
DT   10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT   31-MAY-2011, sequence version 1.
DT   25-MAY-2022, entry version 25.
DE   RecName: Full=Protein FERTILITY RESTORER RF2, mitochondrial {ECO:0000305};
DE   Flags: Precursor;
GN   Name=RF2 {ECO:0000303|PubMed:21265890};
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP   DEVELOPMENTAL STAGE.
RC   STRAIN=cv. Fukuyama, and cv. Owarihatamochi;
RX   PubMed=21265890; DOI=10.1111/j.1365-313x.2010.04427.x;
RA   Itabashi E., Iwata N., Fujii S., Kazama T., Toriyama K.;
RT   "The fertility restorer gene, Rf2, for Lead Rice-type cytoplasmic male
RT   sterility of rice encodes a mitochondrial glycine-rich protein.";
RL   Plant J. 65:359-367(2011).
CC   -!- FUNCTION: Restores fertility in rice varieties with LD-type cytoplasmic
CC       male sterility (CMS) (PubMed:21265890). CMS is caused by genetic
CC       incompatibility between nuclei and mitochondria within male
CC       reproductive organs (PubMed:21265890). Corresponds to the functional
CC       allele of RF2, which is dependent of the presence of ILE-78 in the
CC       japonica cultivars Fukuyama and Owarihatamochi (AC F1SZ42), and indica
CC       cultivar Kasalath (AC F1SZ41) (PubMed:21265890). Non-functional RF2
CC       alleles are found in japonica cultivars Taichung 65 and Nipponbare (AC
CC       F1SZ44), where ILE-78 is replaced by THR-78 (PubMed:21265890).
CC       {ECO:0000269|PubMed:21265890}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:21265890}.
CC   -!- DEVELOPMENTAL STAGE: Specifically expressed during anther development,
CC       from the uninucleate pollen stage to the tricellular pollen stage, with
CC       the highest expression at the tricellular pollen stage.
CC       {ECO:0000269|PubMed:21265890}.
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DR   EMBL; AB583698; BAJ84580.1; -; Genomic_DNA.
DR   EMBL; AB583699; BAJ84581.1; -; Genomic_DNA.
DR   AlphaFoldDB; F1SZ42; -.
DR   SMR; F1SZ42; -.
DR   GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR   InterPro; IPR040299; RF2K-like.
DR   PANTHER; PTHR34938; PTHR34938; 1.
PE   2: Evidence at transcript level;
KW   Mitochondrion; Transit peptide.
FT   TRANSIT         1..52
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           53..152
FT                   /note="Protein FERTILITY RESTORER RF2, mitochondrial"
FT                   /id="PRO_0000445236"
FT   REGION          52..99
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        52..67
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   152 AA;  16144 MW;  557211F4896BD021 CRC64;
     MSTLVTCSLP GAVTTHASTR RFGGSQFQTS QASCISFKRE VSAKAVLRSV RCNATQTQSA
     QRKSSTATVK RSDPKGKIQG PKLDDGSGGF PPFRFGKGGG GGGGGGGGSN YFGGFLLFTC
     VLLLDYLKEF EKNLIARRQR AGYDANNDMF QQ