ID   RF2N_ORYSJ              Reviewed;         152 AA.
AC   F1SZ44; Q0E239;
DT   10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT   31-MAY-2011, sequence version 1.
DT   25-MAY-2022, entry version 39.
DE   RecName: Full=Protein FERTILITY RESTORER RF2, mitochondrial {ECO:0000305};
DE   Flags: Precursor;
GN   Name=RF2 {ECO:0000303|PubMed:21265890};
GN   OrderedLocusNames=Os02g0274000 {ECO:0000312|EMBL:BAF08449.1},
GN   LOC_Os02g17380 {ECO:0000305};
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP   DEVELOPMENTAL STAGE.
RC   STRAIN=cv. Taichung 65;
RX   PubMed=21265890; DOI=10.1111/j.1365-313x.2010.04427.x;
RA   Itabashi E., Iwata N., Fujii S., Kazama T., Toriyama K.;
RT   "The fertility restorer gene, Rf2, for Lead Rice-type cytoplasmic male
RT   sterility of rice encodes a mitochondrial glycine-rich protein.";
RL   Plant J. 65:359-367(2011).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
CC   -!- FUNCTION: Non-functional allele of the RF2 fertility restorer of rice
CC       varieties with LD-type cytoplasmic male sterility (CMS)
CC       (PubMed:21265890). Non-functional RF2 alleles are found in japonica
CC       cultivars Taichung 65 and Nipponbare (AC F1SZ44), and is due to the
CC       presence of THR-78 which replaces ILE-78 in the functional allele
CC       (PubMed:21265890). Functional allele is found in the japonica cultivars
CC       Fukuyama and Owarihatamochi (AC F1SZ42), and indica cultivar Kasalath
CC       (AC F1SZ41) (PubMed:21265890). {ECO:0000269|PubMed:21265890}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:21265890}.
CC   -!- DEVELOPMENTAL STAGE: Specifically expressed during anther development,
CC       from the uninucleate pollen stage to the tricellular pollen stage, with
CC       the highest expression at the tricellular pollen stage.
CC       {ECO:0000269|PubMed:21265890}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAF08449.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=BAS78078.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AB583700; BAJ84582.1; -; Genomic_DNA.
DR   EMBL; AP008208; BAF08449.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP014958; BAS78078.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_015622837.1; XM_015767351.1.
DR   AlphaFoldDB; F1SZ44; -.
DR   STRING; 4530.OS02T0274000-01; -.
DR   EnsemblPlants; Os02t0274000-03; Os02t0274000-03; Os02g0274000.
DR   GeneID; 4328996; -.
DR   Gramene; Os02t0274000-03; Os02t0274000-03; Os02g0274000.
DR   KEGG; osa:4328996; -.
DR   eggNOG; ENOG502S1SV; Eukaryota.
DR   HOGENOM; CLU_104365_2_1_1; -.
DR   OrthoDB; 1592924at2759; -.
DR   Proteomes; UP000000763; Chromosome 2.
DR   Proteomes; UP000059680; Chromosome 2.
DR   GO; GO:0009507; C:chloroplast; IBA:GO_Central.
DR   GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR   GO; GO:0009658; P:chloroplast organization; IBA:GO_Central.
DR   GO; GO:0010027; P:thylakoid membrane organization; IBA:GO_Central.
DR   InterPro; IPR040299; RF2K-like.
DR   PANTHER; PTHR34938; PTHR34938; 1.
PE   2: Evidence at transcript level;
KW   Mitochondrion; Reference proteome; Transit peptide.
FT   TRANSIT         1..52
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           53..152
FT                   /note="Protein FERTILITY RESTORER RF2, mitochondrial"
FT                   /id="PRO_0000445238"
FT   REGION          52..101
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        52..73
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   152 AA;  16132 MW;  55720BC7A071E308 CRC64;
     MSTLVTCSLP GAVTTHASTR RFGGSQFQTS QASCISFKRE VSAKAVLRSV RCNATQTQSA
     QRKSSTATVK RSDPKGKTQG PKLDDGSGGF PPFRFGKGGG GGGGGGGGSN YFGGFLLFTC
     VLLLDYLKEF EKNLIARRQR AGYDANNDMF QQ