ID   RF2_AERHH               Reviewed;         365 AA.
AC   A0KNK6;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-DEC-2006, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Peptide chain release factor 2 {ECO:0000255|HAMAP-Rule:MF_00094};
DE            Short=RF-2 {ECO:0000255|HAMAP-Rule:MF_00094};
GN   Name=prfB {ECO:0000255|HAMAP-Rule:MF_00094}; OrderedLocusNames=AHA_3368;
OS   Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / BCRC
OS   13018 / CCUG 14551 / JCM 1027 / KCTC 2358 / NCIMB 9240 / NCTC 8049).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
OC   Aeromonadaceae; Aeromonas.
OX   NCBI_TaxID=380703;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 7966 / DSM 30187 / BCRC 13018 / CCUG 14551 / JCM 1027 / KCTC
RC   2358 / NCIMB 9240 / NCTC 8049;
RX   PubMed=16980456; DOI=10.1128/jb.00621-06;
RA   Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H.,
RA   Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S.,
RA   Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F.;
RT   "Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades.";
RL   J. Bacteriol. 188:8272-8282(2006).
CC   -!- FUNCTION: Peptide chain release factor 2 directs the termination of
CC       translation in response to the peptide chain termination codons UGA and
CC       UAA. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF2. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000255|HAMAP-Rule:MF_00094}.
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DR   EMBL; CP000462; ABK35994.1; -; Genomic_DNA.
DR   RefSeq; WP_011707133.1; NC_008570.1.
DR   RefSeq; YP_857857.1; NC_008570.1.
DR   AlphaFoldDB; A0KNK6; -.
DR   SMR; A0KNK6; -.
DR   STRING; 380703.AHA_3368; -.
DR   EnsemblBacteria; ABK35994; ABK35994; AHA_3368.
DR   GeneID; 64359904; -.
DR   KEGG; aha:AHA_3368; -.
DR   PATRIC; fig|380703.7.peg.3367; -.
DR   eggNOG; COG1186; Bacteria.
DR   HOGENOM; CLU_220733_2_1_6; -.
DR   OMA; YVFHPYQ; -.
DR   Proteomes; UP000000756; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00094; Rel_fac_2; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004374; PrfB.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00020; prfB; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..365
FT                   /note="Peptide chain release factor 2"
FT                   /id="PRO_1000004970"
FT   MOD_RES         252
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00094"
SQ   SEQUENCE   365 AA;  41064 MW;  7829D2C3975EAB0D CRC64;
     MFEVNPVLNK LKELSERTEL LRGYLDYDAK KERLEEVNAE LEQPDVWNEP ERAQALGKER
     VALENVVGTI DTLTQGADDV AMLVELAVEG EDEDTFNEAV AEADVLETKL VDLEFRRMFS
     GQHDPSDCYI DIQSGSGGTE AQDWANMVLR MYLRWGDAHG YKPELIECSE GDVAGIKSAT
     IKFTGEYAFG WLRTETGVHR LVRKSPFDSG GRRHTSFCSV FVYPEIDDDI DIEINPADLR
     IDVYRASGAG GQHVNRTESA VRITHIPTGV VVQCQNDRSQ HKNKDQCMKQ LKAKLYELEI
     QKQNAEKQAL EETKSDIGWG SQIRSYVLDD ARIKDLRTGV ETRNTQAVLD GDLDKFIEAS
     LKSGL