ID   RF2_AERS4               Reviewed;         365 AA.
AC   A4SJK1;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-MAY-2007, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Peptide chain release factor 2 {ECO:0000255|HAMAP-Rule:MF_00094};
DE            Short=RF-2 {ECO:0000255|HAMAP-Rule:MF_00094};
GN   Name=prfB {ECO:0000255|HAMAP-Rule:MF_00094}; OrderedLocusNames=ASA_0941;
OS   Aeromonas salmonicida (strain A449).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
OC   Aeromonadaceae; Aeromonas.
OX   NCBI_TaxID=382245;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=A449;
RX   PubMed=18801193; DOI=10.1186/1471-2164-9-427;
RA   Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J.,
RA   Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C.,
RA   Brown L.L.;
RT   "The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into
RT   the evolution of a fish pathogen.";
RL   BMC Genomics 9:427-427(2008).
CC   -!- FUNCTION: Peptide chain release factor 2 directs the termination of
CC       translation in response to the peptide chain termination codons UGA and
CC       UAA. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF2. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000255|HAMAP-Rule:MF_00094}.
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DR   EMBL; CP000644; ABO89073.1; -; Genomic_DNA.
DR   AlphaFoldDB; A4SJK1; -.
DR   SMR; A4SJK1; -.
DR   STRING; 382245.ASA_0941; -.
DR   EnsemblBacteria; ABO89073; ABO89073; ASA_0941.
DR   KEGG; asa:ASA_0941; -.
DR   eggNOG; COG1186; Bacteria.
DR   HOGENOM; CLU_220733_2_1_6; -.
DR   OMA; YVFHPYQ; -.
DR   Proteomes; UP000000225; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00094; Rel_fac_2; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004374; PrfB.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00020; prfB; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis.
FT   CHAIN           1..365
FT                   /note="Peptide chain release factor 2"
FT                   /id="PRO_1000004971"
FT   MOD_RES         252
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00094"
SQ   SEQUENCE   365 AA;  41084 MW;  E858E521023506AC CRC64;
     MFEVNPVLNK LKELSERTEL LRGYFDYDAK KERLEEVNAE LEQPDVWNEP ERAQALGKER
     VSLENVVGTI DVLTQGAEDV EMLVSLAVEG EDEDTFNEAV AEADVLETKL VDLEFRRMFS
     GQHDGSDCYI DIQSGSGGTE AQDWANMVLR MYLRWGDAHG YKPELIELSD GDVAGIKSAT
     VKFTGEYAFG WLRTETGVHR LVRKSPFDSG GRRHTSFCSV FVYPEIDDDI DIEINPADLR
     IDVYRASGAG GQHVNRTESA VRITHIPTGV VVQCQNDRSQ HKNKDQCMKQ LKAKLYELEI
     QKQNAEKQAL EETKSDIGWG SQIRSYVLDD ARIKDLRTGV ETRNTQAVLD GDLDKFIEAS
     LKSGL