ID   RF2_AROAE               Reviewed;         367 AA.
AC   Q5NZU2;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   04-JAN-2005, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Peptide chain release factor 2 {ECO:0000255|HAMAP-Rule:MF_00094};
DE            Short=RF-2 {ECO:0000255|HAMAP-Rule:MF_00094};
GN   Name=prfB {ECO:0000255|HAMAP-Rule:MF_00094}; OrderedLocusNames=AZOSEA32970;
GN   ORFNames=ebA5809;
OS   Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales;
OC   Rhodocyclaceae; Aromatoleum.
OX   NCBI_TaxID=76114;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=EbN1;
RX   PubMed=15551059; DOI=10.1007/s00203-004-0742-9;
RA   Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F.,
RA   Reinhardt R.;
RT   "The genome sequence of an anaerobic aromatic-degrading denitrifying
RT   bacterium, strain EbN1.";
RL   Arch. Microbiol. 183:27-36(2005).
CC   -!- FUNCTION: Peptide chain release factor 2 directs the termination of
CC       translation in response to the peptide chain termination codons UGA and
CC       UAA. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF2. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000255|HAMAP-Rule:MF_00094}.
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DR   EMBL; CR555306; CAI09422.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q5NZU2; -.
DR   SMR; Q5NZU2; -.
DR   STRING; 76114.ebA5809; -.
DR   PRIDE; Q5NZU2; -.
DR   EnsemblBacteria; CAI09422; CAI09422; ebA5809.
DR   KEGG; eba:ebA5809; -.
DR   eggNOG; COG1186; Bacteria.
DR   HOGENOM; CLU_220733_0_0_4; -.
DR   OMA; YVFHPYQ; -.
DR   Proteomes; UP000006552; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00094; Rel_fac_2; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004374; PrfB.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00020; prfB; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..367
FT                   /note="Peptide chain release factor 2"
FT                   /id="PRO_0000166801"
FT   MOD_RES         254
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00094"
SQ   SEQUENCE   367 AA;  41546 MW;  86CD09177333C984 CRC64;
     MEAERLNAIA QQLDDLRARG RELRRYLDYD EKSSKLEEVT RALEDPAVWN NAEKAQELGK
     EKRQLEDVVH NLRDIESLSS DLKDLFDLAE AEDDEDTLAA VEADIGELQA KVHALEFRRM
     FSNPMDPNPC FIEIQAGAGG TEAQDWAGML ERMYLRYCER KGFNVELMEE SEGEVAGIKG
     ATIKVSGDYA YGFLRTETGI HRLVRKSPFD SNARRHTSFS SVFVYPEVDD SIQIDINPAD
     LRIDTYRASG AGGQHINKTD SAVRITHEPT GVVVQCQNDR SQHKNKAEAM SMLKARLYEL
     ELRKRQSEQQ KLEDSKSDIG WGHQIRSYVL DQSRIKDLRT NFEVGNTQAV LDGDLDDFIA
     ASLKQGV