RF2_CAMJ8
ID RF2_CAMJ8 Reviewed; 365 AA.
AC A8FNC2;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2007, sequence version 1.
DT 03-AUG-2022, entry version 73.
DE RecName: Full=Peptide chain release factor 2 {ECO:0000255|HAMAP-Rule:MF_00094};
DE Short=RF-2 {ECO:0000255|HAMAP-Rule:MF_00094};
GN Name=prfB {ECO:0000255|HAMAP-Rule:MF_00094}; OrderedLocusNames=C8J_1361;
OS Campylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC
OS 11828).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC Campylobacteraceae; Campylobacter.
OX NCBI_TaxID=407148;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=81116 / NCTC 11828;
RX PubMed=17873037; DOI=10.1128/jb.01404-07;
RA Pearson B.M., Gaskin D.J.H., Segers R.P.A.M., Wells J.M., Nuijten P.J.M.,
RA van Vliet A.H.M.;
RT "The complete genome sequence of Campylobacter jejuni strain 81116
RT (NCTC11828).";
RL J. Bacteriol. 189:8402-8403(2007).
CC -!- FUNCTION: Peptide chain release factor 2 directs the termination of
CC translation in response to the peptide chain termination codons UGA and
CC UAA. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00094}.
CC -!- PTM: Methylated by PrmC. Methylation increases the termination
CC efficiency of RF2. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC family. {ECO:0000255|HAMAP-Rule:MF_00094}.
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DR EMBL; CP000814; ABV52959.1; -; Genomic_DNA.
DR RefSeq; WP_002867022.1; NC_009839.1.
DR AlphaFoldDB; A8FNC2; -.
DR SMR; A8FNC2; -.
DR KEGG; cju:C8J_1361; -.
DR HOGENOM; CLU_036856_6_0_7; -.
DR OMA; YVFHPYQ; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00094; Rel_fac_2; 1.
DR InterPro; IPR005139; PCRF.
DR InterPro; IPR000352; Pep_chain_release_fac_I.
DR InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR InterPro; IPR004374; PrfB.
DR Pfam; PF03462; PCRF; 1.
DR Pfam; PF00472; RF-1; 1.
DR SMART; SM00937; PCRF; 1.
DR SUPFAM; SSF75620; SSF75620; 1.
DR TIGRFAMs; TIGR00020; prfB; 1.
DR PROSITE; PS00745; RF_PROK_I; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Methylation; Protein biosynthesis.
FT CHAIN 1..365
FT /note="Peptide chain release factor 2"
FT /id="PRO_1000071268"
FT MOD_RES 251
FT /note="N5-methylglutamine"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00094"
SQ SEQUENCE 365 AA; 41038 MW; 6B80E8026851921B CRC64;
MDNYEFSELL KTLKNKVGNI ASIIKPENIQ TRLKEIEELE NSPSFWSDVK QAGIIGKEKT
KITNLLKNYE NAFNALNDAS ELFDLANSEN DTETLEALFN DAPKLEDTIT SLEISMLLSG
ENDGKNAIVS IHPGAGGTES NDWASILYRM YLRFCEREGF KVETLDFQEG EEAGLKDVSF
LVKGENAYGY LKAENGIHRL VRTSPFDSAG RRHTSFSSVM VSPELDDDIE IEIEEKDIRI
DYYRASGAGG QHVNKTESAV RITHFPTGIV VQCQNDRSQH KNKATAFKML KSRLYELELM
KQQDSANAGE KSEIGWGHQI RSYVLFPYQQ VKDNRSGEAF SQVDNILDGD IKKMIEGVLI
ALKAE