RF2_CAUVC
ID RF2_CAUVC Reviewed; 371 AA.
AC Q9A752;
DT 01-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Peptide chain release factor 2 {ECO:0000255|HAMAP-Rule:MF_00094};
DE Short=RF-2 {ECO:0000255|HAMAP-Rule:MF_00094};
GN Name=prfB {ECO:0000255|HAMAP-Rule:MF_00094}; OrderedLocusNames=CC_1874;
OS Caulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales;
OC Caulobacteraceae; Caulobacter.
OX NCBI_TaxID=190650;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 19089 / CB15;
RX PubMed=11259647; DOI=10.1073/pnas.061029298;
RA Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E.,
RA Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R.,
RA Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B.,
RA DeBoy R.T., Dodson R.J., Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F.,
RA Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R.,
RA Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O.,
RA Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M.;
RT "Complete genome sequence of Caulobacter crescentus.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001).
CC -!- FUNCTION: Peptide chain release factor 2 directs the termination of
CC translation in response to the peptide chain termination codons UGA and
CC UAA. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00094}.
CC -!- PTM: Methylated by PrmC. Methylation increases the termination
CC efficiency of RF2. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC family. {ECO:0000255|HAMAP-Rule:MF_00094}.
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DR EMBL; AE005673; AAK23849.1; -; Genomic_DNA.
DR PIR; E87481; E87481.
DR RefSeq; NP_420681.1; NC_002696.2.
DR AlphaFoldDB; Q9A752; -.
DR SMR; Q9A752; -.
DR STRING; 190650.CC_1874; -.
DR EnsemblBacteria; AAK23849; AAK23849; CC_1874.
DR KEGG; ccr:CC_1874; -.
DR PATRIC; fig|190650.5.peg.1891; -.
DR eggNOG; COG1186; Bacteria.
DR HOGENOM; CLU_036856_6_0_5; -.
DR OMA; YVFHPYQ; -.
DR BioCyc; CAULO:CC1874-MON; -.
DR Proteomes; UP000001816; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00094; Rel_fac_2; 1.
DR InterPro; IPR005139; PCRF.
DR InterPro; IPR000352; Pep_chain_release_fac_I.
DR InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR InterPro; IPR004374; PrfB.
DR Pfam; PF03462; PCRF; 1.
DR Pfam; PF00472; RF-1; 1.
DR SMART; SM00937; PCRF; 1.
DR SUPFAM; SSF75620; SSF75620; 1.
DR TIGRFAMs; TIGR00020; prfB; 1.
DR PROSITE; PS00745; RF_PROK_I; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Methylation; Protein biosynthesis; Reference proteome.
FT CHAIN 1..371
FT /note="Peptide chain release factor 2"
FT /id="PRO_0000166810"
FT MOD_RES 247
FT /note="N5-methylglutamine"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00094"
SQ SEQUENCE 371 AA; 40922 MW; F66090AC5D3FEB44 CRC64;
MSRPLRPTSS SPWDCSGGVF DWDVALRKLD ELNARVEDPT LWDRPSEAQA VSRERANLAA
KVEAVQSIER DLKDALEYAE LAEMESDEDS LNDARAQLKS LKERAGRAEL EALLSGEADG
NDCYVEINSG AGGTESCDWA GILLRMYTRW ANAHGMTTEL IEETDGDQAG IKSATLLVKG
ANAYGWLKTE AGVHRLVRIS PYDSSARRHT SFASAWVYPV VDDNIEIEIN PSDVRTDTYR
ASGAGGQHIN KTDSAVRLTH IPTGIAVACQ AGRSQHQNRE EAWKMLRARL YEAELQKREA
AQQALEDQKT DIGWGHQIRS YVLQPYQMVK DLRTNVETSD TQGVLDGDLD AFMAASLAQR
VGHTRDGGEA S