RF2_CHLAD
ID RF2_CHLAD Reviewed; 367 AA.
AC B8G607;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 1.
DT 25-MAY-2022, entry version 62.
DE RecName: Full=Peptide chain release factor 2 {ECO:0000255|HAMAP-Rule:MF_00094};
DE Short=RF-2 {ECO:0000255|HAMAP-Rule:MF_00094};
GN Name=prfB {ECO:0000255|HAMAP-Rule:MF_00094}; OrderedLocusNames=Cagg_2879;
OS Chloroflexus aggregans (strain MD-66 / DSM 9485).
OC Bacteria; Chloroflexi; Chloroflexia; Chloroflexales; Chloroflexineae;
OC Chloroflexaceae; Chloroflexus.
OX NCBI_TaxID=326427;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MD-66 / DSM 9485;
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Pitluck S., Foster B., Larimer F., Land M., Hauser L., Kyrpides N.,
RA Mikhailova N., Bryant D.A., Richardson P.;
RT "Complete sequence of Chloroflexus aggregans DSM 9485.";
RL Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Peptide chain release factor 2 directs the termination of
CC translation in response to the peptide chain termination codons UGA and
CC UAA. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00094}.
CC -!- PTM: Methylated by PrmC. Methylation increases the termination
CC efficiency of RF2. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC family. {ECO:0000255|HAMAP-Rule:MF_00094}.
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DR EMBL; CP001337; ACL25740.1; -; Genomic_DNA.
DR AlphaFoldDB; B8G607; -.
DR SMR; B8G607; -.
DR STRING; 326427.Cagg_2879; -.
DR EnsemblBacteria; ACL25740; ACL25740; Cagg_2879.
DR KEGG; cag:Cagg_2879; -.
DR eggNOG; COG1186; Bacteria.
DR HOGENOM; CLU_221244_3_1_0; -.
DR OMA; YVFHPYQ; -.
DR Proteomes; UP000002508; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00094; Rel_fac_2; 1.
DR InterPro; IPR005139; PCRF.
DR InterPro; IPR000352; Pep_chain_release_fac_I.
DR InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR InterPro; IPR004374; PrfB.
DR Pfam; PF03462; PCRF; 1.
DR Pfam; PF00472; RF-1; 1.
DR SMART; SM00937; PCRF; 1.
DR SUPFAM; SSF75620; SSF75620; 1.
DR TIGRFAMs; TIGR00020; prfB; 1.
DR PROSITE; PS00745; RF_PROK_I; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Methylation; Protein biosynthesis.
FT CHAIN 1..367
FT /note="Peptide chain release factor 2"
FT /id="PRO_1000193549"
FT MOD_RES 250
FT /note="N5-methylglutamine"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00094"
SQ SEQUENCE 367 AA; 41732 MW; 397BD987267D91E5 CRC64;
MLAELHEELE TIRERFANLR GHLDLAAKQA EIEQLEVRAS DPELWNTPRV AQELMQRLTR
LKEEVALWND LDHRMTSLAE LIELAEQEGD ESLAADLAAE LRAVQREVAQ RELEILLSGP
YDDRDAFLSI QAGMGGTDAQ DWAAMLLRMY TRWAERRGYT VNLIDLSEGE EAGIKSATIE
IRGPYAYGYA RAEAGVHRLI RLSPFNAAHT RQTSFARVEV MPEVDDAPEV EIKPEDLRID
VFRSGGHGGQ GVNTTDSAVR ITHLPTGIVV TCQNERSQIQ NRETALRVLR ARLLERELQR
QAEERARLRG EYREAAFGNQ MRTYYLHPST LVKDHRTDYE TSNVQAVLDG EIDPFIEAFL
RANVRES