ID   RF2_CHLT2               Reviewed;         368 AA.
AC   B0B832;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Peptide chain release factor 2 {ECO:0000255|HAMAP-Rule:MF_00094};
DE            Short=RF-2 {ECO:0000255|HAMAP-Rule:MF_00094};
GN   Name=prfB {ECO:0000255|HAMAP-Rule:MF_00094}; OrderedLocusNames=CTL0719;
OS   Chlamydia trachomatis serovar L2 (strain 434/Bu / ATCC VR-902B).
OC   Bacteria; Chlamydiae; Chlamydiales; Chlamydiaceae;
OC   Chlamydia/Chlamydophila group; Chlamydia.
OX   NCBI_TaxID=471472;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=434/Bu / ATCC VR-902B;
RX   PubMed=18032721; DOI=10.1101/gr.7020108;
RA   Thomson N.R., Holden M.T.G., Carder C., Lennard N., Lockey S.J., Marsh P.,
RA   Skipp P., O'Connor C.D., Goodhead I., Norbertzcak H., Harris B., Ormond D.,
RA   Rance R., Quail M.A., Parkhill J., Stephens R.S., Clarke I.N.;
RT   "Chlamydia trachomatis: genome sequence analysis of lymphogranuloma
RT   venereum isolates.";
RL   Genome Res. 18:161-171(2008).
CC   -!- FUNCTION: Peptide chain release factor 2 directs the termination of
CC       translation in response to the peptide chain termination codons UGA and
CC       UAA. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF2. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000255|HAMAP-Rule:MF_00094}.
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DR   EMBL; AM884176; CAP04158.1; -; Genomic_DNA.
DR   RefSeq; YP_001654791.1; NC_010287.1.
DR   AlphaFoldDB; B0B832; -.
DR   SMR; B0B832; -.
DR   EnsemblBacteria; CAP04158; CAP04158; CTL0719.
DR   KEGG; ctb:CTL0719; -.
DR   PATRIC; fig|471472.4.peg.771; -.
DR   HOGENOM; CLU_221953_0_0_0; -.
DR   OMA; YVFHPYQ; -.
DR   Proteomes; UP000000795; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00094; Rel_fac_2; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004374; PrfB.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00020; prfB; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis.
FT   CHAIN           1..368
FT                   /note="Peptide chain release factor 2"
FT                   /id="PRO_1000093536"
FT   MOD_RES         250
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00094"
SQ   SEQUENCE   368 AA;  42402 MW;  FE417985F4150A9E CRC64;
     MHENFDKRLE VLLEGLALTR RSLDPEGKEN ELKELEQQAV QDGFWDDVAR AGKISERIAR
     LKQQLSEFNE LKNKVSTIQF FLEDEESSKD LEMQKELEKE FVFCEKKITE WETLRLLSGE
     LDRNSCFLSI NAGAGGTESC DWVEMVLRMY MRWASSHSWR VEVIDRLDGE VAGIKHITLK
     LVGEYAYGYA KAESGVHRLV RISPFDSNAK RHTSFASVEV FPEIDDKIEV EIHPGDIRID
     TYRSSGAGGQ HVNVTDSAVR ITHFPTGIVV SCQNERSQIQ NREACMNMLR ARIYQKLLQE
     RLEKQNIDRK NKKEISWGSQ IRNYVFQPYT LVKDVRTGYE VGNIQAMMDG ELLDAFIKAY
     LVDYGEIT