ID   RF2_CHLTA               Reviewed;         369 AA.
AC   Q3KLN9;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Peptide chain release factor 2 {ECO:0000255|HAMAP-Rule:MF_00094};
DE            Short=RF-2 {ECO:0000255|HAMAP-Rule:MF_00094};
GN   Name=prfB {ECO:0000255|HAMAP-Rule:MF_00094}; OrderedLocusNames=CTA_0501;
OS   Chlamydia trachomatis serovar A (strain ATCC VR-571B / DSM 19440 / HAR-13).
OC   Bacteria; Chlamydiae; Chlamydiales; Chlamydiaceae;
OC   Chlamydia/Chlamydophila group; Chlamydia.
OX   NCBI_TaxID=315277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC VR-571B / DSM 19440 / HAR-13;
RX   PubMed=16177312; DOI=10.1128/iai.73.10.6407-6418.2005;
RA   Carlson J.H., Porcella S.F., McClarty G., Caldwell H.D.;
RT   "Comparative genomic analysis of Chlamydia trachomatis oculotropic and
RT   genitotropic strains.";
RL   Infect. Immun. 73:6407-6418(2005).
CC   -!- FUNCTION: Peptide chain release factor 2 directs the termination of
CC       translation in response to the peptide chain termination codons UGA and
CC       UAA. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF2. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000255|HAMAP-Rule:MF_00094}.
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DR   EMBL; CP000051; AAX50733.1; -; Genomic_DNA.
DR   RefSeq; WP_010725203.1; NC_007429.1.
DR   AlphaFoldDB; Q3KLN9; -.
DR   SMR; Q3KLN9; -.
DR   EnsemblBacteria; AAX50733; AAX50733; CTA_0501.
DR   KEGG; cta:CTA_0501; -.
DR   HOGENOM; CLU_221953_0_0_0; -.
DR   OMA; YVFHPYQ; -.
DR   Proteomes; UP000002532; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00094; Rel_fac_2; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004374; PrfB.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00020; prfB; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis.
FT   CHAIN           1..369
FT                   /note="Peptide chain release factor 2"
FT                   /id="PRO_1000004983"
FT   MOD_RES         251
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00094"
SQ   SEQUENCE   369 AA;  42608 MW;  475431B8EECFACC0 CRC64;
     MHENFDKRLE LLLEGLALTR RSLFDPEGKE NELKELEQQA VQDGFWDDVV RAGKISERIA
     RLKQQLSEFN ELKNKVSAIQ FFLEDEESSK DLEMQKELEK EFVFCEKKIT EWETLRLLSG
     ELDRNSCFLS INAGAGGTES CDWVEMLLRM YMRWASSHSW RIEVIDRLDG EVAGIKHITL
     KLVGEYAYGY AKAESGVHRL VRISPFDSNA KRHTSFASVE VFPEIDDKIE VEIRPGDIRI
     DTYRSSGAGG QHVNVTDSAV RITHFPTGIV VSCQNERSQI QNREACMNML RARIYQKLLQ
     ERLEKQNIDR KNKKEISWGS QIRNYVFQPY TLVKDVRTGY EVGNIQAMMD GELLDAFIKA
     YLVDYGEIT