ID   RF2_CLOPS               Reviewed;         364 AA.
AC   Q0SR12;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Peptide chain release factor 2 {ECO:0000255|HAMAP-Rule:MF_00094};
DE            Short=RF-2 {ECO:0000255|HAMAP-Rule:MF_00094};
GN   Name=prfB {ECO:0000255|HAMAP-Rule:MF_00094}; OrderedLocusNames=CPR_2138;
OS   Clostridium perfringens (strain SM101 / Type A).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=289380;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SM101 / Type A;
RX   PubMed=16825665; DOI=10.1101/gr.5238106;
RA   Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R., DeBoy R.T.,
RA   Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C., Haft D.H.,
RA   Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A.,
RA   Khouri H., Dimitrov G.I., Watkins K.L., Mulligan S., Benton J., Radune D.,
RA   Fisher D.J., Atkins H.S., Hiscox T., Jost B.H., Billington S.J.,
RA   Songer J.G., McClane B.A., Titball R.W., Rood J.I., Melville S.B.,
RA   Paulsen I.T.;
RT   "Skewed genomic variability in strains of the toxigenic bacterial pathogen,
RT   Clostridium perfringens.";
RL   Genome Res. 16:1031-1040(2006).
RN   [2]
RP   SEQUENCE REVISION TO 27.
RA   Shrivastava S., Brinkac L.M., Dodson R.J., Harkins D.M., Durkin A.S.,
RA   Sutton G.;
RL   Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Peptide chain release factor 2 directs the termination of
CC       translation in response to the peptide chain termination codons UGA and
CC       UAA. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF2. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000255|HAMAP-Rule:MF_00094}.
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DR   EMBL; CP000312; ABG86573.2; -; Genomic_DNA.
DR   AlphaFoldDB; Q0SR12; -.
DR   SMR; Q0SR12; -.
DR   EnsemblBacteria; ABG86573; ABG86573; CPR_2138.
DR   KEGG; cpr:CPR_2138; -.
DR   OMA; YVFHPYQ; -.
DR   Proteomes; UP000001824; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00094; Rel_fac_2; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004374; PrfB.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00020; prfB; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis.
FT   CHAIN           1..364
FT                   /note="Peptide chain release factor 2"
FT                   /id="PRO_1000202708"
FT   MOD_RES         252
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00094"
SQ   SEQUENCE   364 AA;  41166 MW;  D046BF7898528745 CRC64;
     MIMELENQLS KLHELKNNLK EMGASLDLAS LEKKSAELEL KMQEAGFWDD VQKAQEVTQE
     AKRVKDKIDK FKNLNERIDD VEVLKELMEE NDEETAKEII SEVKALSKEI DTLKIETILS
     GEYDRNNAIL TLHTGVGGSD ANDWTEMLLR MYTRWCEKKG YSLETIDYLP GDEAGVKSVT
     LKVKGEFAYG YLKAEKGIHR LVRISPFNAN GKRQTSFASV EVLPELTSDQ DIEINPVDLR
     IDTYRAGGAG GQHVNKTESA VRITHIPTGI VVQCQNERSQ FSNRDTAMGM LKSKLIELKE
     RAHKEKIEDL TGELKDMGWG SQIRSYVFHP YSMVKDHRTN VETSNVNGVM GGDIDNFIIA
     YLNS