ID   RF2_JANMA               Reviewed;         367 AA.
AC   A6SXF4;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Peptide chain release factor 2 {ECO:0000255|HAMAP-Rule:MF_00094};
DE            Short=RF-2 {ECO:0000255|HAMAP-Rule:MF_00094};
GN   Name=prfB {ECO:0000255|HAMAP-Rule:MF_00094}; OrderedLocusNames=mma_1261;
OS   Janthinobacterium sp. (strain Marseille) (Minibacterium massiliensis).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Oxalobacteraceae; Janthinobacterium.
OX   NCBI_TaxID=375286;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Marseille;
RX   PubMed=17722982; DOI=10.1371/journal.pgen.0030138;
RA   Audic S., Robert C., Campagna B., Parinello H., Claverie J.-M., Raoult D.,
RA   Drancourt M.;
RT   "Genome analysis of Minibacterium massiliensis highlights the convergent
RT   evolution of water-living bacteria.";
RL   PLoS Genet. 3:1454-1463(2007).
CC   -!- FUNCTION: Peptide chain release factor 2 directs the termination of
CC       translation in response to the peptide chain termination codons UGA and
CC       UAA. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF2. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000255|HAMAP-Rule:MF_00094}.
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DR   EMBL; CP000269; ABR88973.1; -; Genomic_DNA.
DR   AlphaFoldDB; A6SXF4; -.
DR   SMR; A6SXF4; -.
DR   STRING; 375286.mma_1261; -.
DR   PRIDE; A6SXF4; -.
DR   EnsemblBacteria; ABR88973; ABR88973; mma_1261.
DR   KEGG; mms:mma_1261; -.
DR   eggNOG; COG1186; Bacteria.
DR   HOGENOM; CLU_220733_0_0_4; -.
DR   OMA; YVFHPYQ; -.
DR   Proteomes; UP000006388; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00094; Rel_fac_2; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004374; PrfB.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00020; prfB; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..367
FT                   /note="Peptide chain release factor 2"
FT                   /id="PRO_1000004991"
FT   MOD_RES         254
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00094"
SQ   SEQUENCE   367 AA;  41635 MW;  AB8DCFC1C2E67280 CRC64;
     MEAERLNSLE SLLADLTTRA TELRRYLDFD VKSEKLDQVN GELEDPDVWN DQKRAQDLGK
     EKKSLEAIVH TLIKIDSELN DARDLFEMAR EEKDEDTIVA IENDAQELLK LVEGMEFRRM
     FSNPMDANNC FIDIQAGAGG TEAQDWASML LRQYLRYCER KGFKVEILEQ SDGEVAGIKT
     ATLKVEGDYA YGFLRTETGV HRLVRKSPFD SANGRHTSFS SLFVYPEVDD SIEIDINPAD
     VRIDTYRASG AGGQHINKTD SAVRLTHGPS GIVVQCQNDR SQHRNKAEAW DMLKAKLFEL
     EMRNRMSEQQ KLEDSKTDVG WGHQIRSYVL DQSRIKDLRT NFETGNTKAV LDGDLDDFIA
     ASLKQGV