ID   RF2_LACPL               Reviewed;         377 AA.
AC   Q88YL5; F9ULW8; F9ULW9;
DT   31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   14-DEC-2011, sequence version 2.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Peptide chain release factor 2 {ECO:0000255|HAMAP-Rule:MF_00094};
DE            Short=RF-2 {ECO:0000255|HAMAP-Rule:MF_00094};
GN   Name=prfB {ECO:0000255|HAMAP-Rule:MF_00094}; OrderedLocusNames=lp_0741;
OS   Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
OS   (Lactobacillus plantarum).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactiplantibacillus.
OX   NCBI_TaxID=220668;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX   PubMed=12566566; DOI=10.1073/pnas.0337704100;
RA   Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P.,
RA   Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J.,
RA   Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M.,
RA   Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M.,
RA   Siezen R.J.;
RT   "Complete genome sequence of Lactobacillus plantarum WCFS1.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX   PubMed=22156394; DOI=10.1128/jb.06275-11;
RA   Siezen R.J., Francke C., Renckens B., Boekhorst J., Wels M.,
RA   Kleerebezem M., van Hijum S.A.;
RT   "Complete resequencing and reannotation of the Lactobacillus plantarum
RT   WCFS1 genome.";
RL   J. Bacteriol. 194:195-196(2012).
CC   -!- FUNCTION: Peptide chain release factor 2 directs the termination of
CC       translation in response to the peptide chain termination codons UGA and
CC       UAA. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF2. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- MISCELLANEOUS: The gene for this protein contains a UGA in-frame
CC       termination codon after Leu-30; a naturally occurring frameshift
CC       enables complete translation of RF-2. This provides a mechanism for the
CC       protein to regulate its own production (Probable). {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CCC78207.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC       Sequence=CCC78207.1; Type=Frameshift; Evidence={ECO:0000305};
CC       Sequence=CCC78208.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AL935263; CCC78207.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AL935263; CCC78208.1; ALT_FRAME; Genomic_DNA.
DR   RefSeq; YP_004888721.1; NC_004567.2.
DR   RefSeq; YP_004888722.1; NC_004567.2.
DR   AlphaFoldDB; Q88YL5; -.
DR   SMR; Q88YL5; -.
DR   STRING; 220668.lp_0741; -.
DR   EnsemblBacteria; CCC78207; CCC78207; lp_0740.
DR   EnsemblBacteria; CCC78208; CCC78208; lp_0741.
DR   KEGG; lpl:lp_0740; -.
DR   KEGG; lpl:lp_0741; -.
DR   PATRIC; fig|220668.9.peg.623; -.
DR   eggNOG; COG1186; Bacteria.
DR   HOGENOM; CLU_221244_2_2_9; -.
DR   PhylomeDB; Q88YL5; -.
DR   BioCyc; LPLA220668:G1GW0-643-MON; -.
DR   Proteomes; UP000000432; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00094; Rel_fac_2; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004374; PrfB.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00020; prfB; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis; Reference proteome;
KW   Ribosomal frameshifting.
FT   CHAIN           1..377
FT                   /note="Peptide chain release factor 2"
FT                   /id="PRO_0000166824"
FT   MOD_RES         257
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00094"
SQ   SEQUENCE   377 AA;  42652 MW;  FDD4DB1783085BDC CRC64;
     MKEGSFVELS EYKHLIEEMQ SAVDDFRGSL DLDALNESIQ ENEARMAEPG FWDDQAAAQK
     VIDENNVLKG KYDTFKQLAD EVGDLAVAYE LLSEEPDAEM QAEFETDFQH AEHDLQQYRL
     NLLLDGPYDR NNAILEIHPG AGGTESQDWG AMLLRMYTRW AASHNFKVET VDYQAGDEAG
     IKSVTLLISG HNAYGYLRSE KGVHRLVRIS PFDAAGRRHT SFASVDVMPE LDDTVDVDIR
     PEDLKIDVYR ASGAGGQHVN KTSSAVRITH VPTGIVVASQ AQRSQLQNRQ TALNMLRAKL
     YEREEEKKAK ERAAIQGEQM DIGWGSQIRS YVFHPYTMVK DHRTNYESHH GQAVMDGDLD
     PFMDAYLQWK LAQRNPQ