RF2_LISIN
ID RF2_LISIN Reviewed; 366 AA.
AC Q927Y4;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2005, sequence version 2.
DT 25-MAY-2022, entry version 103.
DE RecName: Full=Peptide chain release factor 2;
DE Short=RF-2;
GN Name=prfB; OrderedLocusNames=lin2653;
OS Listeria innocua serovar 6a (strain ATCC BAA-680 / CLIP 11262).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria.
OX NCBI_TaxID=272626;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-680 / CLIP 11262;
RX PubMed=11679669; DOI=10.1126/science.1063447;
RA Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F.,
RA Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A.,
RA Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E.,
RA Dominguez-Bernal G., Duchaud E., Durant L., Dussurget O., Entian K.-D.,
RA Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W.,
RA Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U.,
RA Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A.,
RA Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B.,
RA Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N.,
RA Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P.;
RT "Comparative genomics of Listeria species.";
RL Science 294:849-852(2001).
CC -!- FUNCTION: Peptide chain release factor 2 directs the termination of
CC translation in response to the peptide chain termination codons UGA and
CC UAA. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- PTM: Methylated by PrmC. Methylation increases the termination
CC efficiency of RF2 (By similarity). {ECO:0000250}.
CC -!- MISCELLANEOUS: The gene for this protein contains a UGA in-frame
CC termination codon after Leu-24; a naturally occurring frameshift
CC enables complete translation of RF-2. This provides a mechanism for the
CC protein to regulate its own production (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAC97879.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AL596173; CAC97879.1; ALT_INIT; Genomic_DNA.
DR PIR; AG1763; AG1763.
DR AlphaFoldDB; Q927Y4; -.
DR SMR; Q927Y4; -.
DR STRING; 272626.lin2653; -.
DR EnsemblBacteria; CAC97879; CAC97879; CAC97879.
DR KEGG; lin:prfB; -.
DR eggNOG; COG1186; Bacteria.
DR HOGENOM; CLU_036856_6_0_9; -.
DR Proteomes; UP000002513; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00094; Rel_fac_2; 1.
DR InterPro; IPR005139; PCRF.
DR InterPro; IPR000352; Pep_chain_release_fac_I.
DR InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR InterPro; IPR004374; PrfB.
DR Pfam; PF03462; PCRF; 1.
DR Pfam; PF00472; RF-1; 1.
DR SMART; SM00937; PCRF; 1.
DR SUPFAM; SSF75620; SSF75620; 1.
DR TIGRFAMs; TIGR00020; prfB; 1.
DR PROSITE; PS00745; RF_PROK_I; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Methylation; Protein biosynthesis; Ribosomal frameshifting.
FT CHAIN 1..366
FT /note="Peptide chain release factor 2"
FT /id="PRO_0000166827"
FT MOD_RES 251
FT /note="N5-methylglutamine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 366 AA; 42036 MW; 030FD3EC78406FDF CRC64;
MELAEIRNEL EKTAQQIKDF RGSLDLDSME VRIAELEDQM LDPNFWNDQQ AAQKVINESN
GYKETYQAFH ALEEEQESME ISLELLKEEA DEDLQAELEK DIKAYVATIS EFELKLMLSD
PYDKNNAILE LHPGAGGTES QDWGSMLLRM YQRWSEKKGF KVEMLDYQAG DEAGIKSVTL
LIKGHNAYGY LKAEKGVHRL VRISPFDSSG RRHTSFVSVD VMPELDDEIE IEVRTEDLKI
DTYRATGAGG QHINTTDSAV RMTHIPSGIV VTCQSERSQL KNREQAMKML KTKLYQKEQE
EKERELAEIR GEQKEIGWGS QIRSYVFHPY SMVKDHRTNY ETGNIQAVMD GDLDDFINAY
LRSRIG