ID   RF2_MYCA1               Reviewed;         370 AA.
AC   A0QJS1;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Peptide chain release factor 2 {ECO:0000255|HAMAP-Rule:MF_00094};
DE            Short=RF-2 {ECO:0000255|HAMAP-Rule:MF_00094};
GN   Name=prfB {ECO:0000255|HAMAP-Rule:MF_00094}; OrderedLocusNames=MAV_4006;
OS   Mycobacterium avium (strain 104).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium avium complex (MAC).
OX   NCBI_TaxID=243243;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=104;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Peptide chain release factor 2 directs the termination of
CC       translation in response to the peptide chain termination codons UGA and
CC       UAA. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF2. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000255|HAMAP-Rule:MF_00094}.
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DR   EMBL; CP000479; ABK69191.1; -; Genomic_DNA.
DR   RefSeq; WP_011725809.1; NC_008595.1.
DR   AlphaFoldDB; A0QJS1; -.
DR   SMR; A0QJS1; -.
DR   EnsemblBacteria; ABK69191; ABK69191; MAV_4006.
DR   GeneID; 66695211; -.
DR   KEGG; mav:MAV_4006; -.
DR   HOGENOM; CLU_036856_6_0_11; -.
DR   OMA; YVFHPYQ; -.
DR   OrthoDB; 928964at2; -.
DR   Proteomes; UP000001574; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00094; Rel_fac_2; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004374; PrfB.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00020; prfB; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis.
FT   CHAIN           1..370
FT                   /note="Peptide chain release factor 2"
FT                   /id="PRO_1000004998"
FT   MOD_RES         252
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00094"
SQ   SEQUENCE   370 AA;  41277 MW;  2DA34F76C5AAA3B5 CRC64;
     MEPDRQADIA ALDSTLTTVE RVLDVDGLRA RIEKLEHEAS DPQLWDDQAR AQRVTSELSH
     AQGELRRIEE LRARLDDLPV LYELAAEEGE GAAEALAEAD AELKALRADI EATEVRTLLS
     GEYDEREALV TIRSGAGGVD AADWAEMLMR MYIRWAEQHK YPVEVFDTSY AEEAGIKSAT
     FAVHAPFAYG TLSVEQGTHR LVRISPFDNQ SRRQTSFAEV EVLPVVETTD HIDIPEGDVR
     VDVYRSSGPG GQSVNTTDSA VRLTHIPTGI VVTCQNEKSQ LQNKISAMRV LQAKLLERKR
     SEERAELDAL KGEGGSSWGN QMRSYVLHPY QMVKDLRTEY EVGNPAAVLD GDIDGFLEAG
     IRWRNRKDDE