ID   RF2_NOCFA               Reviewed;         372 AA.
AC   Q5YR52;
DT   27-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=Peptide chain release factor 2 {ECO:0000255|HAMAP-Rule:MF_00094};
DE            Short=RF-2 {ECO:0000255|HAMAP-Rule:MF_00094};
GN   Name=prfB {ECO:0000255|HAMAP-Rule:MF_00094}; OrderedLocusNames=NFA_44880;
OS   Nocardia farcinica (strain IFM 10152).
OC   Bacteria; Actinobacteria; Corynebacteriales; Nocardiaceae; Nocardia.
OX   NCBI_TaxID=247156;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IFM 10152;
RX   PubMed=15466710; DOI=10.1073/pnas.0406410101;
RA   Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K.,
RA   Shiba T., Hattori M.;
RT   "The complete genomic sequence of Nocardia farcinica IFM 10152.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004).
CC   -!- FUNCTION: Peptide chain release factor 2 directs the termination of
CC       translation in response to the peptide chain termination codons UGA and
CC       UAA. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF2. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000255|HAMAP-Rule:MF_00094}.
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DR   EMBL; AP006618; BAD59339.1; -; Genomic_DNA.
DR   RefSeq; WP_011211023.1; NC_006361.1.
DR   AlphaFoldDB; Q5YR52; -.
DR   SMR; Q5YR52; -.
DR   STRING; 247156.NFA_44880; -.
DR   EnsemblBacteria; BAD59339; BAD59339; NFA_44880.
DR   GeneID; 61135094; -.
DR   KEGG; nfa:NFA_44880; -.
DR   eggNOG; COG1186; Bacteria.
DR   HOGENOM; CLU_036856_6_0_11; -.
DR   OMA; YVFHPYQ; -.
DR   Proteomes; UP000006820; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00094; Rel_fac_2; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004374; PrfB.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00020; prfB; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..372
FT                   /note="Peptide chain release factor 2"
FT                   /id="PRO_0000166836"
FT   MOD_RES         253
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00094"
SQ   SEQUENCE   372 AA;  41970 MW;  55324F464568581D CRC64;
     MHPDVSADLA ELDATLKTVE SVLDIEELRR RIDELEHQAA DPDLWNDQDH AQRVTSELSH
     AQGELRRVED LRRRLEDLPV LYELAEGEEG EARTAALEEA DAERAALHSD VEAMEVRTLL
     SGEYDKREAL VNIRSGAGGV DAADWAEMLM RMYIRWADRH GYPVEVYDTS YAEEAGIKSA
     TFAVKTPYAY GTLSVEMGTH RLVRISPFDN QGRRQTSFAE VEVLPVVETT DHIEVPETEI
     RVDVYRSSGP GGQSVNTTDS AVRITHIPTG IVVTCQNEKS QLQNKISAMR VLQAKLLERK
     RQEERAEMDA LKTNEGASWG NQMRSYVLHP YQMVKDLRTN YEVNNPSAVL NGDIDGFIES
     GIRWRMRESQ AS