ID   RF2_RIPO1               Reviewed;         365 AA.
AC   B7K0A6;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Peptide chain release factor 2 {ECO:0000255|HAMAP-Rule:MF_00094};
DE            Short=RF-2 {ECO:0000255|HAMAP-Rule:MF_00094};
GN   Name=prfB {ECO:0000255|HAMAP-Rule:MF_00094};
GN   OrderedLocusNames=PCC8801_3422;
OS   Rippkaea orientalis (strain PCC 8801) (Cyanothece sp. (strain PCC 8801)).
OC   Bacteria; Cyanobacteria; Oscillatoriophycideae; Chroococcales;
OC   Aphanothecaceae; Rippkaea; Rippkaea orientalis.
OX   NCBI_TaxID=41431;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 8801;
RX   PubMed=21972240; DOI=10.1128/mbio.00214-11;
RA   Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H.,
RA   Sherman L.A., Pakrasi H.B.;
RT   "Novel metabolic attributes of the genus Cyanothece, comprising a group of
RT   unicellular nitrogen-fixing Cyanobacteria.";
RL   MBio 2:E214-E214(2011).
CC   -!- FUNCTION: Peptide chain release factor 2 directs the termination of
CC       translation in response to the peptide chain termination codons UGA and
CC       UAA. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF2. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000255|HAMAP-Rule:MF_00094}.
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DR   EMBL; CP001287; ACK67390.1; -; Genomic_DNA.
DR   AlphaFoldDB; B7K0A6; -.
DR   SMR; B7K0A6; -.
DR   STRING; 41431.PCC8801_3422; -.
DR   EnsemblBacteria; ACK67390; ACK67390; PCC8801_3422.
DR   KEGG; cyp:PCC8801_3422; -.
DR   eggNOG; COG1186; Bacteria.
DR   HOGENOM; CLU_220736_0_0_3; -.
DR   OMA; YVFHPYQ; -.
DR   Proteomes; UP000008204; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00094; Rel_fac_2; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004374; PrfB.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00020; prfB; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..365
FT                   /note="Peptide chain release factor 2"
FT                   /id="PRO_1000117257"
FT   MOD_RES         252
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00094"
SQ   SEQUENCE   365 AA;  41144 MW;  7B15B4FF77B8BD8A CRC64;
     MEITELKREI QQIAARLGKT QDYLDLPALN ANIQDLEQIA AQPDFWDNQE TAQKTLQQLN
     DLKSSVEEYH QWMGQLEDLK AIAELLELEE DATLNEEAEA NLTQLNHELD RWELQRLLSG
     IYDSKGAVLT INAGAGGTDA QDWAEMLLRM YTRWGEQQGY KVHLTEISEG DEAGIKSATL
     EIEGRYAYGY LKGEKGTHRL VRISPFNANG KRQTSFAGIE VMPALEEEDL KVEIPEKDLE
     ITTTRSGGKG GQNVNKVETA VRVVHLPTGI AVRCTQERSQ LQNKEKALAL LKAKLLIIAQ
     EQRAQAIAEI RGDMVEAAWG NQIRNYVFHP YQMVKDVRTN TETTDVNGVM DGKLDLFIES
     YLRSN