RF2_STAA2
ID RF2_STAA2 Reviewed; 369 AA.
AC A6TZN3;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 21-AUG-2007, sequence version 1.
DT 25-MAY-2022, entry version 79.
DE RecName: Full=Peptide chain release factor 2 {ECO:0000255|HAMAP-Rule:MF_00094};
DE Short=RF-2 {ECO:0000255|HAMAP-Rule:MF_00094};
GN Name=prfB {ECO:0000255|HAMAP-Rule:MF_00094};
GN OrderedLocusNames=SaurJH1_0795;
OS Staphylococcus aureus (strain JH1).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=359787;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JH1;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA Hauser L., Kyrpides N., Ivanova N., Tomasz A., Richardson P.;
RT "Complete sequence of chromosome of Staphylococcus aureus subsp. aureus
RT JH1.";
RL Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Peptide chain release factor 2 directs the termination of
CC translation in response to the peptide chain termination codons UGA and
CC UAA. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00094}.
CC -!- PTM: Methylated by PrmC. Methylation increases the termination
CC efficiency of RF2. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC family. {ECO:0000255|HAMAP-Rule:MF_00094}.
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DR EMBL; CP000736; ABR51651.1; -; Genomic_DNA.
DR AlphaFoldDB; A6TZN3; -.
DR SMR; A6TZN3; -.
DR KEGG; sah:SaurJH1_0795; -.
DR HOGENOM; CLU_221244_2_2_9; -.
DR OMA; YVFHPYQ; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00094; Rel_fac_2; 1.
DR InterPro; IPR005139; PCRF.
DR InterPro; IPR000352; Pep_chain_release_fac_I.
DR InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR InterPro; IPR004374; PrfB.
DR Pfam; PF03462; PCRF; 1.
DR Pfam; PF00472; RF-1; 1.
DR SMART; SM00937; PCRF; 1.
DR SUPFAM; SSF75620; SSF75620; 1.
DR TIGRFAMs; TIGR00020; prfB; 1.
DR PROSITE; PS00745; RF_PROK_I; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Methylation; Protein biosynthesis.
FT CHAIN 1..369
FT /note="Peptide chain release factor 2"
FT /id="PRO_1000075530"
FT MOD_RES 252
FT /note="N5-methylglutamine"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00094"
SQ SEQUENCE 369 AA; 42331 MW; 45A2AD406F8ABA07 CRC64;
MELSEIKRNI DKYNQDLTQI RGSLDLENKE TNIQEYEEMM AEPNFWDNQT KAQDIIDKNN
ALKAIVNGYK TLQAEVDDMD ATWDLLQEEF DEEMKEDLEQ EVINFKAKVD EYELQLLLDG
PHDANNAILE LHPGAGGTES QDWANMLFRM YQRYCEKKGF KVETVDYLPG DEAGIKSVTL
LIKGHNAYGY LKAEKGVHRL VRISPFDSSG RRHTSFASCD VIPDFNNDEI EIEINPDDIT
VDTFRASGAG GQHINKTESA IRITHHPSGI VVNNQNERSQ IKNREAAMKM LKSKLYQLKL
EEQAREMAEI RGEQKEIGWG SQIRSYVFHP YSMVKDHRTN EETGKVDAVM DGDIGPFIES
YLRQTMSHD