RF2_STRPG
ID RF2_STRPG Reviewed; 365 AA.
AC A2RFN2;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 06-MAR-2007, sequence version 1.
DT 25-MAY-2022, entry version 76.
DE RecName: Full=Peptide chain release factor 2 {ECO:0000255|HAMAP-Rule:MF_00094};
DE Short=RF-2 {ECO:0000255|HAMAP-Rule:MF_00094};
GN Name=prfB {ECO:0000255|HAMAP-Rule:MF_00094}; OrderedLocusNames=SpyM51333;
OS Streptococcus pyogenes serotype M5 (strain Manfredo).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=160491;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Manfredo;
RX PubMed=17012393; DOI=10.1128/jb.01227-06;
RA Holden M.T.G., Scott A., Cherevach I., Chillingworth T., Churcher C.,
RA Cronin A., Dowd L., Feltwell T., Hamlin N., Holroyd S., Jagels K.,
RA Moule S., Mungall K., Quail M.A., Price C., Rabbinowitsch E., Sharp S.,
RA Skelton J., Whitehead S., Barrell B.G., Kehoe M., Parkhill J.;
RT "Complete genome of acute rheumatic fever-associated serotype M5
RT Streptococcus pyogenes strain Manfredo.";
RL J. Bacteriol. 189:1473-1477(2007).
CC -!- FUNCTION: Peptide chain release factor 2 directs the termination of
CC translation in response to the peptide chain termination codons UGA and
CC UAA. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00094}.
CC -!- PTM: Methylated by PrmC. Methylation increases the termination
CC efficiency of RF2. {ECO:0000255|HAMAP-Rule:MF_00094}.
CC -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC family. {ECO:0000255|HAMAP-Rule:MF_00094}.
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DR EMBL; AM295007; CAM30661.1; -; Genomic_DNA.
DR AlphaFoldDB; A2RFN2; -.
DR SMR; A2RFN2; -.
DR PRIDE; A2RFN2; -.
DR KEGG; spf:SpyM51333; -.
DR HOGENOM; CLU_221244_1_0_9; -.
DR OMA; YVFHPYQ; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00094; Rel_fac_2; 1.
DR InterPro; IPR005139; PCRF.
DR InterPro; IPR000352; Pep_chain_release_fac_I.
DR InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR InterPro; IPR004374; PrfB.
DR Pfam; PF03462; PCRF; 1.
DR Pfam; PF00472; RF-1; 1.
DR SMART; SM00937; PCRF; 1.
DR SUPFAM; SSF75620; SSF75620; 1.
DR TIGRFAMs; TIGR00020; prfB; 1.
DR PROSITE; PS00745; RF_PROK_I; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Methylation; Protein biosynthesis.
FT CHAIN 1..365
FT /note="Peptide chain release factor 2"
FT /id="PRO_1000005014"
FT MOD_RES 249
FT /note="N5-methylglutamine"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00094"
SQ SEQUENCE 365 AA; 41734 MW; 5E3D717BFDD75BA7 CRC64;
MEVAEIRQKI VENKEKLTSF RRSLDLDRLE EEIALLENHM TEPDFWNDNI AAQKTSQELN
ELKGKYDTFH NMQELSDETE LLLEMLDEDD SLKEELEENL MQLDKIMGAY EMTLLLSEPY
DHNNAILEIH PGSGGTEAQD WGDLLLRMYT RFGNANGFKV EVLDYQAGDE AGIKSVTLSF
EGPNAYGLLK SEMGVHRLVR ISPFDSAKRR HTSFASVEVM PELDNTIEVE VRDDDIKMDT
FRSGGAGGQN VNKVSTGVRL THIPTGIVVS STVDRTQYGN RDRAMKMLQA KLYQLEQEKK
AQEVDALKGD KKEITWGSQI RSYVFTPYTM VKDHRTNFEL AQVDKVMDGE INGFIDAYLK
WRIED