RF3_GLOVI
ID RF3_GLOVI Reviewed; 531 AA.
AC Q7NGL0;
DT 15-DEC-2003, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2003, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Peptide chain release factor 3 {ECO:0000255|HAMAP-Rule:MF_00072};
DE Short=RF-3 {ECO:0000255|HAMAP-Rule:MF_00072};
GN Name=prfC {ECO:0000255|HAMAP-Rule:MF_00072}; OrderedLocusNames=glr3159;
OS Gloeobacter violaceus (strain ATCC 29082 / PCC 7421).
OC Bacteria; Cyanobacteria; Gloeobacteria; Gloeobacterales; Gloeobacteraceae;
OC Gloeobacter.
OX NCBI_TaxID=251221;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29082 / PCC 7421;
RX PubMed=14621292; DOI=10.1093/dnares/10.4.137;
RA Nakamura Y., Kaneko T., Sato S., Mimuro M., Miyashita H., Tsuchiya T.,
RA Sasamoto S., Watanabe A., Kawashima K., Kishida Y., Kiyokawa C., Kohara M.,
RA Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Takeuchi C., Yamada M.,
RA Tabata S.;
RT "Complete genome structure of Gloeobacter violaceus PCC 7421, a
RT cyanobacterium that lacks thylakoids.";
RL DNA Res. 10:137-145(2003).
CC -!- FUNCTION: Increases the formation of ribosomal termination complexes
CC and stimulates activities of RF-1 and RF-2. It binds guanine
CC nucleotides and has strong preference for UGA stop codons. It may
CC interact directly with the ribosome. The stimulation of RF-1 and RF-2
CC is significantly reduced by GTP and GDP, but not by GMP.
CC {ECO:0000255|HAMAP-Rule:MF_00072}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00072}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. PrfC subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00072}.
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DR EMBL; BA000045; BAC91100.1; -; Genomic_DNA.
DR RefSeq; NP_926105.1; NC_005125.1.
DR RefSeq; WP_011143151.1; NC_005125.1.
DR AlphaFoldDB; Q7NGL0; -.
DR SMR; Q7NGL0; -.
DR STRING; 251221.35213730; -.
DR EnsemblBacteria; BAC91100; BAC91100; BAC91100.
DR KEGG; gvi:glr3159; -.
DR PATRIC; fig|251221.4.peg.3190; -.
DR eggNOG; COG4108; Bacteria.
DR HOGENOM; CLU_002794_2_1_3; -.
DR InParanoid; Q7NGL0; -.
DR OMA; GFVFKIH; -.
DR OrthoDB; 164090at2; -.
DR PhylomeDB; Q7NGL0; -.
DR Proteomes; UP000000557; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0016150; F:translation release factor activity, codon nonspecific; IBA:GO_Central.
DR GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR GO; GO:0006449; P:regulation of translational termination; IEA:UniProtKB-UniRule.
DR GO; GO:0006415; P:translational termination; IBA:GO_Central.
DR CDD; cd04169; RF3; 1.
DR Gene3D; 3.30.70.3280; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00072; Rel_fac_3; 1.
DR InterPro; IPR035647; EFG_III/V.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR031157; G_TR_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR004548; PrfC.
DR InterPro; IPR032090; RF3_C.
DR InterPro; IPR038467; RF3_dom_3_sf.
DR InterPro; IPR041732; RF3_GTP-bd.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR PANTHER; PTHR43556; PTHR43556; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR Pfam; PF16658; RF3_C; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF50447; SSF50447; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF54980; SSF54980; 1.
DR TIGRFAMs; TIGR00503; prfC; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS00301; G_TR_1; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; GTP-binding; Nucleotide-binding; Protein biosynthesis;
KW Reference proteome.
FT CHAIN 1..531
FT /note="Peptide chain release factor 3"
FT /id="PRO_0000210942"
FT DOMAIN 11..280
FT /note="tr-type G"
FT BINDING 20..27
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00072"
FT BINDING 88..92
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00072"
FT BINDING 142..145
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00072"
SQ SEQUENCE 531 AA; 59736 MW; 1D9148C550A89154 CRC64;
MSNPTLAAEV GRRRTFAIIS HPDAGKTTLT EKLLLYGGAI DMAGSVRARR NQRHATSDWM
AMEQQRGISI TSTVLQFVYR DCQINLLDTP GHQDFSEDTY RTLAAADNAA MLIDAAKGIE
AQTRKLFDVC RMRGIPIFTF INKLDRPGRE PLELLDEIEK VLGIDVYPVN WPIGMGDTFR
GVFDRLEHTV HLFDRTTGGK KMAPVTVGAI GDERMRVLMD EGTYAQIVEE IELLDGVGVP
FDIERVAQGK LTPVFFGSAA NNFGVQLFLD AFIRFASRPG TRSANTGVIS PVAEDFSGFV
FKIQANMDPQ HRDRVAFIRV CSGKFEKDMT VHHTRSGKKV RLSRSLKLFG QERETVEEAY
AGDIVGVINP GTFAIGDTIC LGKPLAFEGI PLFPPEHFAT LRNPNPSKYK QFLKGVTQLR
EEGAVQVLFH QDEAKRDPIL AAVGQLQFDV VRFRLESEYH VETILEPLPW TLARWITAKQ
LEDLETIDWY FDSLGLKDHE GRLVILFKTP WGFQQMSERN PHLQFHEIAP L