AAIM1_CAEEL
ID AAIM1_CAEEL Reviewed; 515 AA.
AC Q5WRS0;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 11-NOV-2015, sequence version 3.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=Protein aaim-1 {ECO:0000305};
DE AltName: Full=Antibacterial and aids invasion by microsporidia 1 protein {ECO:0000303|PubMed:34994689};
DE Flags: Precursor;
GN Name=aaim-1 {ECO:0000303|PubMed:34994689, ECO:0000312|WormBase:T14E8.4};
GN ORFNames=T14E8.4 {ECO:0000312|WormBase:T14E8.4};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP INDUCTION, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF 1-MET--CYS-17.
RX PubMed=34994689; DOI=10.7554/elife.72458;
RA Tamim El Jarkass H., Mok C., Schertzberg M.R., Fraser A.G., Troemel E.R.,
RA Reinke A.W.;
RT "An intestinally secreted host factor promotes microsporidia invasion of C.
RT elegans.";
RL Elife 11:0-0(2022).
CC -!- FUNCTION: Plays a role in promoting resistance to bacterial pathogens
CC such as P.aeruginosa by inhibiting bacterial intestinal colonization.
CC {ECO:0000269|PubMed:34994689}.
CC -!- FUNCTION: (Microbial infection) Promotes infection by microsporidian
CC pathogens such as N.parisii in the early larval stages of development
CC (PubMed:34994689). Involved in ensuring the proper orientation and
CC location of the spore proteins of N.parisii during intestinal cell
CC invasion (PubMed:34994689). {ECO:0000269|PubMed:34994689}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:34994689}.
CC -!- TISSUE SPECIFICITY: Expressed in the terminal bulb of the pharynx and
CC the posterior of the intestine (at protein level) (PubMed:34994689).
CC Expressed by intestinal cells and secreted into the intestinal lumen
CC (at protein level) (PubMed:34994689). {ECO:0000269|PubMed:34994689}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the terminal bulb of the pharynx and
CC the posterior of the intestine throughout development (at protein
CC level) (PubMed:34994689). Expressed in the arcade cells of the pharynx
CC in embryos and in L1 stage larvae (at protein level) (PubMed:34994689).
CC {ECO:0000269|PubMed:34994689}.
CC -!- INDUCTION: Up-regulated following infection by the microsporidian
CC pathogen N.parisii or the bacterial pathogen P.aeruginosa.
CC {ECO:0000269|PubMed:34994689}.
CC -!- DISRUPTION PHENOTYPE: Resistant to infection by the microsporidian
CC pathogen N.parisii and displays a fitness advantage phenotype, whereby
CC there is increased fitness following infection by N.parisii
CC (PubMed:34994689). Fewer invading N.parisii spores following infection
CC at the L1 larval stage of development due to impaired orientation,
CC location and angle of N.parisii spore proteins relative to the host's
CC intestinal apical membrane (PubMed:34994689). Does not affect the
CC ability of N.parisii to invade intestinal cells or N.parisii spore
CC consumption by intestinal cells of L1 stage larvae (PubMed:34994689).
CC Animals are not resistant to infection by N.parisii at the L3 larval
CC stage of development (PubMed:34994689). Reduced survival following
CC infection by the bacteria P.aeruginosa at the L1 and L4 larval stages
CC due to increased accumulation of the bacteria within the intestinal
CC lumen (PubMed:34994689). Reduced survival and increased numbers of
CC N.parisii spore proteins following co-infection with N.parisii and
CC P.aeruginosa (PubMed:34994689). {ECO:0000269|PubMed:34994689}.
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DR EMBL; BX284606; CCD83408.2; -; Genomic_DNA.
DR RefSeq; NP_001024909.3; NM_001029738.4.
DR AlphaFoldDB; Q5WRS0; -.
DR PaxDb; Q5WRS0; -.
DR EnsemblMetazoa; T14E8.4.1; T14E8.4.1; WBGene00043981.
DR GeneID; 3565421; -.
DR KEGG; cel:CELE_T14E8.4; -.
DR UCSC; T14E8.4; c. elegans.
DR CTD; 3565421; -.
DR WormBase; T14E8.4; CE50409; WBGene00043981; aaim-1.
DR eggNOG; ENOG502TG6B; Eukaryota.
DR GeneTree; ENSGT00970000196263; -.
DR HOGENOM; CLU_043714_0_0_1; -.
DR InParanoid; Q5WRS0; -.
DR OMA; EAKLMHT; -.
DR OrthoDB; 708137at2759; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00043981; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0005615; C:extracellular space; IDA:UniProtKB.
DR GO; GO:2000535; P:regulation of entry of bacterium into host cell; IMP:UniProtKB.
PE 1: Evidence at protein level;
KW Glycoprotein; Reference proteome; Secreted; Signal.
FT SIGNAL 1..16
FT /evidence="ECO:0000255"
FT CHAIN 17..515
FT /note="Protein aaim-1"
FT /id="PRO_5005698498"
FT REGION 248..267
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 46
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 127
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 447
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT MUTAGEN 1..17
FT /note="Missing: Promotes infection by the microsporidian
FT pathogen N.parisii as in wild-type."
FT /evidence="ECO:0000269|PubMed:34994689"
SQ SEQUENCE 515 AA; 58205 MW; 26BA69BA923BB439 CRC64;
MRLLFFFSIL YTASLCCQLK DFLPCVMQLS AQKVDFNMNP IEVIFNITTE AKLMHTCRTY
SRILPCFDQK MVQCGKPSEK TQLERGKRLH SYLCAPFSLQ RQKIFLRRSK CIQDVLAEPQ
SSVCNRNDTV FADKLQSCRE MCTRPDCVSK IELSEVSTCT YINIGKKCTA EAAQFFAQMQ
QVLTNKEYPM QCQYDLRKKP ESELKKGLPI ESLVAQTTSS TTYVTVHPPA LPSVIDGVVT
RTSLPIMRRT DPNSKFKPRP TTSQSNGPVI KTVIVDERGA PMNQPTSTQK PKVVHKFLPN
PYTTKNPNTL KNDIIRTTRT IIPVVDKHTY VPWNYKVDAV QVSTLTSALA PVKPTETVIS
SPPVAFNFKL PAEQTSTQPF RVEINWHDDE VKQEPTKAPG VFVSPWYLKT PSHIPPEIEF
ATPTPLISSP LEAVSPILSQ LKSNSLNFTE LGNQANNYFS AALSAFAETK KEMAHNDPWR
TIIDAVAPTI HKFSPDVIPR IREEINRIQP HQQKN
