RF4_ARATH
ID RF4_ARATH Reviewed; 823 AA.
AC Q9ZVT8;
DT 10-AUG-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Putative E3 ubiquitin-protein ligase RF4;
DE EC=2.3.2.27;
DE AltName: Full=RING finger protein 4;
DE AltName: Full=RING-type E3 ubiquitin transferase RF4 {ECO:0000305};
GN Name=RF4; OrderedLocusNames=At1g03365; ORFNames=F15K9.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY ORGANIZATION.
RX PubMed=11983057; DOI=10.1186/gb-2002-3-4-research0016;
RA Kosarev P., Mayer K.F.X., Hardtke C.S.;
RT "Evaluation and classification of RING-finger domains encoded by the
RT Arabidopsis genome.";
RL Genome Biol. 3:RESEARCH0016.1-RESEARCH0016.12(2002).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- DOMAIN: The RING-type zinc finger domain mediates binding to an E2
CC ubiquitin-conjugating enzyme. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the RING-type zinc finger family. {ECO:0000305}.
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DR EMBL; AC005278; AAC72106.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE27564.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM58631.1; -; Genomic_DNA.
DR PIR; D86165; D86165.
DR RefSeq; NP_001184899.1; NM_001197970.2.
DR RefSeq; NP_001321053.1; NM_001331406.1.
DR AlphaFoldDB; Q9ZVT8; -.
DR SMR; Q9ZVT8; -.
DR STRING; 3702.AT1G03365.1; -.
DR PaxDb; Q9ZVT8; -.
DR PRIDE; Q9ZVT8; -.
DR ProteomicsDB; 236992; -.
DR EnsemblPlants; AT1G03365.1; AT1G03365.1; AT1G03365.
DR EnsemblPlants; AT1G03365.2; AT1G03365.2; AT1G03365.
DR GeneID; 10723112; -.
DR Gramene; AT1G03365.1; AT1G03365.1; AT1G03365.
DR Gramene; AT1G03365.2; AT1G03365.2; AT1G03365.
DR KEGG; ath:AT1G03365; -.
DR Araport; AT1G03365; -.
DR TAIR; locus:6530298128; AT1G03365.
DR eggNOG; ENOG502SB0J; Eukaryota.
DR HOGENOM; CLU_012143_0_1_1; -.
DR InParanoid; Q9ZVT8; -.
DR OMA; KPHAIAR; -.
DR OrthoDB; 259830at2759; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q9ZVT8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9ZVT8; baseline and differential.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 3: Inferred from homology;
KW Coiled coil; Metal-binding; Reference proteome; Transferase;
KW Ubl conjugation pathway; Zinc; Zinc-finger.
FT CHAIN 1..823
FT /note="Putative E3 ubiquitin-protein ligase RF4"
FT /id="PRO_0000395977"
FT ZN_FING 768..808
FT /note="RING-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT REGION 24..72
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 224..291
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 432..464
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 536..738
FT /evidence="ECO:0000255"
FT COMPBIAS 25..39
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 224..241
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 252..291
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 432..455
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 823 AA; 91537 MW; 64D007A4E87D0E53 CRC64;
MSIVQKQEEM NGCGLNVDKV EAFTVSPQEK GRKNKRKLAD PSQPNASSLT EFPPYELPSL
KPQNHLSGNG SVGEVSNQLQ VEVSESVEWD DPFACHLEEL LSSNLLTLFL DTMKQLIDLG
YTDDEVLKAV SRCRLYCGGN NLLSNIVNNT LSALKTGDEG AGSGDYVFED LQQLVSYTLV
EMISLIKEVR PSLSTVEAMW RLLMCDLNVL QAFEAEGDGL VSSSKLSDSE SLGAESNPPK
SSDPDNPKPP QSDPQSNRNE PLKFGNFPNT PNSKKTQSSG TTPGKEVCSG STVSCQGMRS
TSFTLVSDEK LVSCRKGRTK KEIAMLRQKS CVEKIRTYSK GSGYKAAKFA SVGSFLLEKR
VKSSSEFVPR NSSSKITAEI GVKVSLAEDS GCFVRKNSKL DSPVVVVDAK GYITALPARS
VKSASKKKTG SESVTLIPSA SEKKSDSSIP STSEKKSGSE SEEKASVSAK LAPDYYAGIP
YDAALGIYVP RDKKDELILK LVPRVNDLQN ELQVWTDWAN QKVKEATGRL LKDQPELKAL
RKEREEAEQY KKEKQLLEEN TRKRLSEMDF ALKNATSQLE KAFNTAHRLE LEQSILKKEM
EAAKIKAVES AESFREAKER GERSLKDIHS WEGQKIMLQE ELKGQREKVT VLQKEVTKAK
NRQNQIEAAL KQERTAKGKL SAQASLIRKE TKELEALGKV EEERIKGKAE TDVKYYIDNI
KRLEREISEL KLKSDYSRII ALKKGSSESK ATKRESLGMP KVKRERECVM CLSEEMSVIF
LPCAHQVLCF KCNQLHEKEG MMDCPSCRGT IHRRIQARFA RSG
