ID   RF4_KLULA               Reviewed;         428 AA.
AC   P09806;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   25-MAY-2022, entry version 62.
DE   RecName: Full=RF4 protein;
GN   Name=RF4;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OG   Plasmid pGKl-1.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 76492 / CBS 2359/152 / CLIB 210;
RX   DOI=10.1007/BF00436963;
RA   Sor F., Fukuhara H.;
RT   "Structure of a linear plasmid of the yeast Kluyveromyces lactis; compact
RT   organization of the killer genome.";
RL   Curr. Genet. 9:147-155(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=6473099; DOI=10.1093/nar/12.15.6011;
RA   Stark M.J.R., Mileham A.J., Romanos M.A., Boyd A.;
RT   "Nucleotide sequence and transcription analysis of a linear DNA plasmid
RT   associated with the killer character of the yeast Kluyveromyces lactis.";
RL   Nucleic Acids Res. 12:6011-6030(1984).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 52735 / 2105-1D;
RX   PubMed=6387625; DOI=10.1093/nar/12.19.7581;
RA   Hishinuma F., Nakamura K., Hirai K., Nishizawa R., Gunge N., Maeda T.;
RT   "Cloning and nucleotide sequences of the linear DNA killer plasmids from
RT   yeast.";
RL   Nucleic Acids Res. 12:7581-7597(1984).
CC   -!- FUNCTION: Not known.
CC   -!- MISCELLANEOUS: The presence of the two linear plasmids, termed pGKl-1
CC       and pGKl-2, in strains of Kluyveromyces lactis confers the killer
CC       phenotype, i.e. production of toxin and resistance to it, to the host
CC       cell.
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DR   EMBL; X07127; CAA30138.1; -; Genomic_DNA.
DR   EMBL; X00762; CAA25335.1; -; Genomic_DNA.
DR   EMBL; X01095; CAA25570.1; -; Genomic_DNA.
DR   PIR; S07916; S07916.
DR   AlphaFoldDB; P09806; -.
DR   PRIDE; P09806; -.
DR   InParanoid; P09806; -.
DR   Proteomes; UP000000598; Plasmid pGKL1.
PE   4: Predicted;
KW   Glycoprotein; Plasmid; Reference proteome.
FT   CHAIN           1..428
FT                   /note="RF4 protein"
FT                   /id="PRO_0000097256"
FT   CARBOHYD        8
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        205
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        344
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   428 AA;  50949 MW;  782F18C51D89BE6C CRC64;
     MCLELDNNLS DYFKACTYAY YSDYENFIKY YDNEELFDED DLNFHLCIGI RNSYNFAVKM
     ENSGLKLTSG ILSSISARYI MSNKVCDFNF KYLPSGIWYP NVPTKETCIY LLEKDPKYSL
     VIKTICVLLG WDDVCDKCDK ICTEVCFRLS IIFNRRSFCE EDTIYNYKIH ENKNILEKVE
     KDKYLLPETI LRNKDTTLEI FFKYNYTSKQ SNIEQIIDDM LYTYIDYSQN TEFGPMDCVS
     VDKSVYYNMV SYKPYLDINI NTVEFNFLNS AYVLKHHKEY PDDLCKKAVL NLLVNDIYDS
     NFVSLYRPFY FYTYKTLNKY MVQKLLIDFP RQRYAILVNY AIMNITEDLE NYSPDIDLIR
     IAYASGSIEF YNIMMNKAKK EGGMCQHLDF ENEKYIFKYH KYEDVDFDNL HKNNGHPLLL
     RATHLNFP