RFA1A_ORYSJ
ID RFA1A_ORYSJ Reviewed; 656 AA.
AC Q6YZ49; A0A0P0VQ40; Q9AVL8;
DT 26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Replication protein A 70 kDa DNA-binding subunit A;
DE Short=OsRPA70a;
DE AltName: Full=Replication factor A protein 1A;
DE AltName: Full=Replication protein A 1A;
GN Name=RPA1A; Synonyms=RPA70A;
GN OrderedLocusNames=Os02g0776800, LOC_Os02g53680;
GN ORFNames=OJ1534_E09.2, OsJ_08582, OSJNBb0013K01.36;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND INDUCTION.
RC STRAIN=cv. Nipponbare;
RX PubMed=11470540; DOI=10.1016/s0378-1119(01)00555-8;
RA Ishibashi T., Kimura S., Furukawa T., Hatanaka M., Hashimoto J.,
RA Sakaguchi K.;
RT "Two types of replication protein A 70 kDa subunit in rice, Oryza sativa:
RT molecular cloning, characterization, and cellular & tissue distribution.";
RL Gene 272:335-343(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP FUNCTION, AND INTERACTION WITH RPA2A.
RX PubMed=15978034; DOI=10.1111/j.1742-4658.2005.04719.x;
RA Ishibashi T., Koga A., Yamamoto T., Uchiyama Y., Mori Y., Hashimoto J.,
RA Kimura S., Sakaguchi K.;
RT "Two types of replication protein A in seed plants.";
RL FEBS J. 272:3270-3281(2005).
RN [8]
RP INTERACTION WITH RPA2B.
RX PubMed=16428324; DOI=10.1093/jb/mvj014;
RA Ishibashi T., Kimura S., Sakaguchi K.;
RT "A higher plant has three different types of RPA heterotrimeric complex.";
RL J. Biochem. 139:99-104(2006).
RN [9]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19812186; DOI=10.1104/pp.109.142877;
RA Chang Y., Gong L., Yuan W., Li X., Chen G., Li X., Zhang Q., Wu C.;
RT "Replication protein A (RPA1a) is required for meiotic and somatic DNA
RT repair but is dispensable for DNA replication and homologous recombination
RT in rice.";
RL Plant Physiol. 151:2162-2173(2009).
CC -!- FUNCTION: Component of the replication protein A complex (RPA) required
CC for DNA recombination, repair and replication. The activity of RPA is
CC mediated by single-stranded DNA binding and protein interactions. Plays
CC an essential role in meiotic and somatic DNA repair, but is dispensable
CC for DNA replication and homologous recombination. Is essential for
CC normal progression through meiosis in pollen mother cells. Is involved
CC in repair of double-strand DNA breaks (DSBs) induced by genotoxic
CC stresses. {ECO:0000269|PubMed:15978034, ECO:0000269|PubMed:19812186}.
CC -!- SUBUNIT: Heterotrimer of RPA1, RPA2 and RPA3 (canonical replication
CC protein A complex) (By similarity). Interacts with RPA2B. {ECO:0000250,
CC ECO:0000269|PubMed:15978034, ECO:0000269|PubMed:16428324}.
CC -!- INTERACTION:
CC Q6YZ49; Q6H7J5: RPA2B; NbExp=4; IntAct=EBI-849583, EBI-849532;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in root tips, roots, shoot apical
CC meristem (SAM), young leaves, flag leaves and ears, and at lower levels
CC in mature leaves. {ECO:0000269|PubMed:11470540}.
CC -!- INDUCTION: Repressed by sucrose starvation.
CC {ECO:0000269|PubMed:11470540}.
CC -!- DISRUPTION PHENOTYPE: Normal phenotype during vegetative growth, but
CC sterility at the reproductive stage. No formation of embryo sac in
CC female meiocytes and abnormal chromosomal fragmentation after anaphase
CC I in male meiocytes. {ECO:0000269|PubMed:19812186}.
CC -!- SIMILARITY: Belongs to the replication factor A protein 1 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB40712.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB042415; BAB40712.1; ALT_INIT; mRNA.
DR EMBL; AP004140; BAD16963.1; -; Genomic_DNA.
DR EMBL; AP005538; BAD17384.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF10201.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS81173.1; -; Genomic_DNA.
DR EMBL; CM000139; EAZ24804.1; -; Genomic_DNA.
DR EMBL; AK072308; BAG92916.1; -; mRNA.
DR EMBL; AK101212; BAG94958.1; -; mRNA.
DR RefSeq; XP_015625818.1; XM_015770332.1.
DR AlphaFoldDB; Q6YZ49; -.
DR SMR; Q6YZ49; -.
DR IntAct; Q6YZ49; 1.
DR STRING; 4530.OS02T0776800-01; -.
DR PaxDb; Q6YZ49; -.
DR PRIDE; Q6YZ49; -.
DR EnsemblPlants; Os02t0776800-01; Os02t0776800-01; Os02g0776800.
DR EnsemblPlants; Os02t0776800-02; Os02t0776800-02; Os02g0776800.
DR GeneID; 4330902; -.
DR Gramene; Os02t0776800-01; Os02t0776800-01; Os02g0776800.
DR Gramene; Os02t0776800-02; Os02t0776800-02; Os02g0776800.
DR KEGG; osa:4330902; -.
DR eggNOG; KOG0851; Eukaryota.
DR HOGENOM; CLU_012393_3_1_1; -.
DR InParanoid; Q6YZ49; -.
DR OMA; VRVTIWG; -.
DR OrthoDB; 1189265at2759; -.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000007752; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR ExpressionAtlas; Q6YZ49; baseline and differential.
DR Genevisible; Q6YZ49; OS.
DR GO; GO:0000785; C:chromatin; IEA:EnsemblPlants.
DR GO; GO:0005662; C:DNA replication factor A complex; IBA:GO_Central.
DR GO; GO:0003684; F:damaged DNA binding; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043047; F:single-stranded telomeric DNA binding; IBA:GO_Central.
DR GO; GO:0051026; P:chiasma assembly; IEA:EnsemblPlants.
DR GO; GO:0006281; P:DNA repair; IMP:UniProtKB.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IBA:GO_Central.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IBA:GO_Central.
DR GO; GO:0007141; P:male meiosis I; IEA:EnsemblPlants.
DR GO; GO:0007140; P:male meiotic nuclear division; IMP:UniProtKB.
DR GO; GO:0051321; P:meiotic cell cycle; IBA:GO_Central.
DR GO; GO:0006289; P:nucleotide-excision repair; IBA:GO_Central.
DR GO; GO:0009555; P:pollen development; IMP:UniProtKB.
DR GO; GO:0007004; P:telomere maintenance via telomerase; IBA:GO_Central.
DR CDD; cd04476; RPA1_DBD_C; 1.
DR CDD; cd04477; RPA1N; 1.
DR Gene3D; 2.40.50.140; -; 4.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR013955; Rep_factor-A_C.
DR InterPro; IPR007199; Rep_factor-A_N.
DR InterPro; IPR031657; REPA_OB_2.
DR InterPro; IPR004591; Rfa1.
DR PANTHER; PTHR23273; PTHR23273; 1.
DR Pfam; PF04057; Rep-A_N; 1.
DR Pfam; PF08646; Rep_fac-A_C; 1.
DR Pfam; PF16900; REPA_OB_2; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SUPFAM; SSF50249; SSF50249; 4.
DR TIGRFAMs; TIGR00617; rpa1; 1.
PE 1: Evidence at protein level;
KW DNA damage; DNA recombination; DNA repair; DNA replication; DNA-binding;
KW Metal-binding; Nucleus; Reference proteome; Zinc; Zinc-finger.
FT CHAIN 1..656
FT /note="Replication protein A 70 kDa DNA-binding subunit A"
FT /id="PRO_0000422620"
FT DNA_BIND 225..307
FT /note="OB"
FT ZN_FING 516..542
FT /note="C4-type"
FT /evidence="ECO:0000250"
FT CONFLICT 435
FT /note="L -> F (in Ref. 1; BAB40712)"
FT /evidence="ECO:0000305"
FT CONFLICT 459
FT /note="S -> F (in Ref. 1; BAB40712)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 656 AA; 72311 MW; B4C4492EAFEC4556 CRC64;
MAMARLTPNG VAAALAGDTN LKPVLQIVEL RGVQVNGAGV TRGERFRAVV SDGTAASSAL
FAAQLSDHAR SGALRRGSIV QLSEYVINEV GPRRIIVILN LEVLVSECEI IGNPTALSET
GSPIPNPTRV EQFNGAPQYG LMAGNSSNTT TKPSDNVPLF QNSMAGNSSN FATRPSDKVP
VFQPTVQPSY RPAPNYKNHG AIMKNEAPAR IIPISALNPY QGRWAIKARV TAKGDIRRYH
NAKGDGKVFS FDLLDSDGGE IRVTCFNALL DRFYEVVEVG KVYVVSRGNL RPAQKNYNHL
NNEWEILLEN GSTVDLCPDE NSSIPTQRFD FRPINEIEDA QNNAILDIIG VVTSVNPCTT
IQRKNGMETQ KRTMNLKDMS GRSVEVTMWG DFCNREGSQL QGMVERGIFP VLAVKAGKVS
DFSGKSVGTI SSTQLFINPD SAEAHSLRQW FDSGGRDAST QSISRDITPG ASRNEIRKTV
AQIKDEGLGM GDKPDWITVK ATVIFFKNES FFYTACPNMI GDRQCNKKVT KSTNGNWTCD
KCDREFEECD YRYLLQFQIQ DHSGTAWVTA FQEAGQELLG CSATELNALK EREDPRFADT
MLNCLFQEYL LRLKVKEESY GDERKVKNTA VKVEKVDPSG ESKFLLDLIS KSSALH
