RFA1B_ORYSJ
ID RFA1B_ORYSJ Reviewed; 630 AA.
AC Q10Q08; A0A0P0VUM6; Q7Y069;
DT 26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2006, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Replication protein A 70 kDa DNA-binding subunit B;
DE Short=OsRPA70b;
DE AltName: Full=Replication factor A protein 1B;
DE AltName: Full=Replication protein A 1B;
GN Name=RPA1B; Synonyms=RPA70B;
GN OrderedLocusNames=Os03g0214100, LOC_Os03g11540; ORFNames=OsJ_09906;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND INDUCTION.
RC STRAIN=cv. Nipponbare;
RX PubMed=11470540; DOI=10.1016/s0378-1119(01)00555-8;
RA Ishibashi T., Kimura S., Furukawa T., Hatanaka M., Hashimoto J.,
RA Sakaguchi K.;
RT "Two types of replication protein A 70 kDa subunit in rice, Oryza sativa:
RT molecular cloning, characterization, and cellular & tissue distribution.";
RL Gene 272:335-343(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [7]
RP FUNCTION, AND INTERACTION WITH RPA2A.
RX PubMed=15978034; DOI=10.1111/j.1742-4658.2005.04719.x;
RA Ishibashi T., Koga A., Yamamoto T., Uchiyama Y., Mori Y., Hashimoto J.,
RA Kimura S., Sakaguchi K.;
RT "Two types of replication protein A in seed plants.";
RL FEBS J. 272:3270-3281(2005).
RN [8]
RP INTERACTION WITH RPA2A.
RX PubMed=16428324; DOI=10.1093/jb/mvj014;
RA Ishibashi T., Kimura S., Sakaguchi K.;
RT "A higher plant has three different types of RPA heterotrimeric complex.";
RL J. Biochem. 139:99-104(2006).
CC -!- FUNCTION: Component of the replication protein A complex (RPA) required
CC for DNA recombination, repair and replication. The activity of RPA is
CC mediated by single-stranded DNA binding and protein interactions.
CC Probably involved in repair of double-strand DNA breaks (DSBs) induced
CC by genotoxic stresses (By similarity). {ECO:0000250,
CC ECO:0000269|PubMed:15978034}.
CC -!- SUBUNIT: Heterotrimer of RPA1, RPA2 and RPA3 (canonical replication
CC protein A complex) (By similarity). Interacts with RPA2A. {ECO:0000250,
CC ECO:0000269|PubMed:15978034, ECO:0000269|PubMed:16428324}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in root tips, roots, shoot apical
CC meristem (SAM) and young leaves, and at lower levels in mature leaves,
CC flag leaves and ears. {ECO:0000269|PubMed:11470540}.
CC -!- INDUCTION: Repressed by sucrose starvation.
CC {ECO:0000269|PubMed:11470540}.
CC -!- SIMILARITY: Belongs to the replication factor A protein 1 family.
CC {ECO:0000305}.
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DR EMBL; AB111916; BAC77529.1; -; mRNA.
DR EMBL; DP000009; ABF94627.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF11283.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS82952.1; -; Genomic_DNA.
DR EMBL; CM000140; EAZ26052.1; -; Genomic_DNA.
DR RefSeq; XP_015628911.1; XM_015773425.1.
DR AlphaFoldDB; Q10Q08; -.
DR SMR; Q10Q08; -.
DR STRING; 4530.OS03T0214100-01; -.
DR PaxDb; Q10Q08; -.
DR PRIDE; Q10Q08; -.
DR EnsemblPlants; Os03t0214100-01; Os03t0214100-01; Os03g0214100.
DR GeneID; 4332052; -.
DR Gramene; Os03t0214100-01; Os03t0214100-01; Os03g0214100.
DR KEGG; osa:4332052; -.
DR eggNOG; KOG0851; Eukaryota.
DR HOGENOM; CLU_012393_3_1_1; -.
DR InParanoid; Q10Q08; -.
DR OMA; YRLLINM; -.
DR OrthoDB; 1189265at2759; -.
DR PlantReactome; R-OSA-9645850; Activation of pre-replication complex.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000007752; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR Genevisible; Q10Q08; OS.
DR GO; GO:0005662; C:DNA replication factor A complex; IDA:UniProtKB.
DR GO; GO:0003684; F:damaged DNA binding; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043047; F:single-stranded telomeric DNA binding; IBA:GO_Central.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IBA:GO_Central.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IBA:GO_Central.
DR GO; GO:0051321; P:meiotic cell cycle; IBA:GO_Central.
DR GO; GO:0006289; P:nucleotide-excision repair; IBA:GO_Central.
DR GO; GO:0007004; P:telomere maintenance via telomerase; IBA:GO_Central.
DR CDD; cd04476; RPA1_DBD_C; 1.
DR CDD; cd04477; RPA1N; 1.
DR DisProt; DP01086; -.
DR Gene3D; 2.40.50.140; -; 4.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR013955; Rep_factor-A_C.
DR InterPro; IPR007199; Rep_factor-A_N.
DR InterPro; IPR031657; REPA_OB_2.
DR InterPro; IPR004591; Rfa1.
DR PANTHER; PTHR23273; PTHR23273; 1.
DR Pfam; PF04057; Rep-A_N; 1.
DR Pfam; PF08646; Rep_fac-A_C; 1.
DR Pfam; PF16900; REPA_OB_2; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SUPFAM; SSF50249; SSF50249; 4.
DR TIGRFAMs; TIGR00617; rpa1; 1.
PE 1: Evidence at protein level;
KW DNA damage; DNA recombination; DNA repair; DNA replication; DNA-binding;
KW Metal-binding; Nucleus; Reference proteome; Zinc; Zinc-finger.
FT CHAIN 1..630
FT /note="Replication protein A 70 kDa DNA-binding subunit B"
FT /id="PRO_0000422621"
FT DNA_BIND 200..282
FT /note="OB"
FT ZN_FING 496..516
FT /note="C4-type"
FT /evidence="ECO:0000255"
FT CONFLICT 269
FT /note="N -> D (in Ref. 1; BAC77529)"
FT /evidence="ECO:0000305"
FT CONFLICT 411
FT /note="V -> L (in Ref. 1; BAC77529)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 630 AA; 69604 MW; 6593FE3B68125C20 CRC64;
MDSDAAPSVT PGAVAFVLEN ASPDAATGVP VPEIVLQVVD LKPIGTRFTF LASDGKDKIK
TMLLTQLAPE VRSGNIQNLG VIRVLDYTCN TIGEKQEKVL IITKLEVVFK ALDSEIKCEA
EKQEEKPAIL LSPKEESVVL SKPTNAPPLP PVVLKPKQEV KSASQIVNEQ RGNAAPAARL
AMTRRVHPLI SLNPYQGNWI IKVRVTSKGN LRTYKNARGE GCVFNVELTD VDGTQIQATM
FNEAAKKFYP MFELGKVYYI SKGSLRVANK QFKTVHNDYE MTLNENAVVE EAEGETFIPQ
IQYNFVKIDQ LGPYVGGREL VDVIGVVQSV SPTLSVRRKI DNETIPKRDI VVADDSSKTV
TISLWNDLAT TTGQELLDMV DSAPIIAIKS LKVSDFQGLS LSTVGRSTIV VNPDLPEAEQ
LRAWYDSEGK GTSMASIGSD MGASRVGGAR SMYSDRVFLS HITSDPNLGQ DKPVFFSLNA
YISLIKPDQT MWYRACKTCN KKVTEAIGSG YWCEGCQKND AECSLRYIMV IKVSDPTGEA
WLSLFNDQAE RIVGCSADEL DRIRKEEGDD SYLLKLKEAT WVPHLFRVSV TQNEYMNEKR
QRITVRSEAP VDHAAEAKYM LEEIAKLTGC
