RFA1_CAEBR
ID RFA1_CAEBR Reviewed; 658 AA.
AC O97472;
DT 20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Probable replication factor A 73 kDa subunit {ECO:0000250|UniProtKB:Q19537};
DE AltName: Full=RP-A p73;
DE AltName: Full=Replication factor A protein 1;
DE Short=RF-A protein 1;
GN Name=rpa-1 {ECO:0000312|EMBL:CAP31894.1}; ORFNames=CBG13026;
OS Caenorhabditis briggsae.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6238;
RN [1] {ECO:0000312|EMBL:CAA10310.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10224256; DOI=10.1093/genetics/152.1.221;
RA Dufourcq P., Chanal P., Vicaire S., Camut E., Quintin S., den Boer B.B.W.,
RA Bosher J.M., Labouesse M.;
RT "lir-2, lir-1 and lin-26 encode a new class of zinc-finger proteins and are
RT organized in two overlapping operons both in Caenorhabditis elegans and in
RT Caenorhabditis briggsae.";
RL Genetics 152:221-235(1999).
RN [2] {ECO:0000312|EMBL:CAP31894.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AF16 {ECO:0000312|EMBL:CAP31894.1};
RX PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P.,
RA Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W.,
RA Hillier L.W., Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A.,
RA Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E.,
RA Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K.,
RA Durbin R.M., Waterston R.H.;
RT "The genome sequence of Caenorhabditis briggsae: a platform for comparative
RT genomics.";
RL PLoS Biol. 1:166-192(2003).
CC -!- FUNCTION: As part of the heterotrimeric replication protein A complex
CC (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates,
CC that form during DNA replication or upon DNA stress. It prevents their
CC reannealing and in parallel, recruits and activates different proteins
CC and complexes involved in DNA metabolism. Thereby, it plays an
CC essential role both in DNA replication and the cellular response to DNA
CC damage. {ECO:0000250|UniProtKB:P27694}.
CC -!- SUBUNIT: Component of the heterotrimeric canonical replication protein
CC A complex (RPA). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q19537}.
CC -!- SIMILARITY: Belongs to the replication factor A protein 1 family.
CC {ECO:0000255}.
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DR EMBL; AJ131134; CAA10310.1; -; Genomic_DNA.
DR EMBL; AJ131634; CAA10466.1; -; Genomic_DNA.
DR EMBL; HE600938; CAP31894.1; -; Genomic_DNA.
DR RefSeq; XP_002630573.1; XM_002630527.1.
DR AlphaFoldDB; O97472; -.
DR SMR; O97472; -.
DR STRING; 6238.CBG13026; -.
DR EnsemblMetazoa; CBG13026.1; CBG13026.1; WBGene00033864.
DR GeneID; 8572089; -.
DR KEGG; cbr:CBG_13026; -.
DR CTD; 8572089; -.
DR WormBase; CBG13026; CBP03074; WBGene00033864; Cbr-rpa-1.
DR eggNOG; KOG0851; Eukaryota.
DR HOGENOM; CLU_012393_2_1_1; -.
DR InParanoid; O97472; -.
DR OMA; YRLLINM; -.
DR OrthoDB; 1189265at2759; -.
DR Proteomes; UP000008549; Chromosome II.
DR GO; GO:0005662; C:DNA replication factor A complex; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IEA:EnsemblMetazoa.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0003684; F:damaged DNA binding; IBA:GO_Central.
DR GO; GO:0019899; F:enzyme binding; IEA:EnsemblMetazoa.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003697; F:single-stranded DNA binding; ISS:UniProtKB.
DR GO; GO:0043047; F:single-stranded telomeric DNA binding; IBA:GO_Central.
DR GO; GO:0006260; P:DNA replication; ISS:UniProtKB.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IBA:GO_Central.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IBA:GO_Central.
DR GO; GO:0051321; P:meiotic cell cycle; IBA:GO_Central.
DR GO; GO:0006289; P:nucleotide-excision repair; IBA:GO_Central.
DR GO; GO:0051096; P:positive regulation of helicase activity; IEA:EnsemblMetazoa.
DR GO; GO:0110039; P:positive regulation of nematode male tail tip morphogenesis; IEA:EnsemblMetazoa.
DR GO; GO:0007004; P:telomere maintenance via telomerase; IBA:GO_Central.
DR CDD; cd04476; RPA1_DBD_C; 1.
DR Gene3D; 2.40.50.140; -; 3.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR013955; Rep_factor-A_C.
DR InterPro; IPR031657; REPA_OB_2.
DR InterPro; IPR004591; Rfa1.
DR PANTHER; PTHR23273; PTHR23273; 1.
DR Pfam; PF08646; Rep_fac-A_C; 1.
DR Pfam; PF16900; REPA_OB_2; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SUPFAM; SSF50249; SSF50249; 3.
DR TIGRFAMs; TIGR00617; rpa1; 1.
PE 3: Inferred from homology;
KW DNA replication; DNA-binding; Metal-binding; Nucleus; Reference proteome;
KW Zinc; Zinc-finger.
FT CHAIN 1..658
FT /note="Probable replication factor A 73 kDa subunit"
FT /id="PRO_0000361044"
FT DNA_BIND 236..326
FT /note="OB"
FT /evidence="ECO:0000255"
FT ZN_FING 518..539
FT /note="C4-type"
FT /evidence="ECO:0000250|UniProtKB:Q19537, ECO:0000255"
FT REGION 134..155
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 169..222
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 187..201
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 658 AA; 73427 MW; A919B58C8061A6E4 CRC64;
MTSIKISSDV FNKYHTNGKL RLSTGYVQEA LEKQGYPGHD GVVQILKGKE DIGEQMGHGF
TYRIRICDGI FQYNTLVSAD IDDQIKREAE HLVEGAIIAI TNLSTFSQGA GIKTSFLITG
YTLLSRYHQT LSAPEVKPRS HSGNPAEHHG YRPNIVVEDV WPEAESITSE FQENMSNPPA
AKMPKRESGG EASHNRVPAP EPHRSRAPPP PARRGPSNTE RGVIPIAMVT PYVNNFRIHG
MVSRKEDIKN IPAKNMKIFN FEITDSNGDT IRCTAFNETA ESFHSTITEN LSYYLSGGSV
RQANKKFNNT GHDYEITLRN DSVVEAGGEL LAAPKLNLKR VSLAEIAGHC GEMIDVLVIV
EKMDAEATEF TSKAGKTLTK REMELIDESQ ALVRLTLWGD EAIKANVDDY HGKVIAFKGV
IPREFNGGYS LGTGSGTRII PVPEISGVSE LYDWYTTEKP HSELKLISQT SGGMSEAPRT
IAGLQEMQFG KDSDKGDYAS VKAMITRINP NSALYKGCAS EGCQKKVIES DGEYRCEKCN
KSMNKFKWLY MMQFELSDET GQVYVTAFGD SAAKVVGKTA QEVGDLKDEN LNEYNATFER
LQFVPKMWRL RCKMETYNEE VRQKMTVFSV EEVNQDKYIE NLKELIEQMK GIEDEGSY
