RFA2B_ARATH
ID RFA2B_ARATH Reviewed; 278 AA.
AC Q8LFJ8; Q9M8T4;
DT 31-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Replication protein A 32 kDa subunit B;
DE Short=AtRPA32B;
DE Short=RP-A p32 B;
DE AltName: Full=DNA replication protein A2 subunit B;
DE AltName: Full=Replication factor A protein 2 B;
DE Short=AtRPA2 B;
DE Short=RF-A protein 2 B;
DE AltName: Full=Replicon protein A2 B;
GN Name=RPA2B; Synonyms=RPA32B; OrderedLocusNames=At3g02920;
GN ORFNames=F13E7.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. C24, and cv. Columbia;
RX PubMed=16326925; DOI=10.1105/tpc.105.037507;
RA Xia R., Wang J., Liu C., Wang Y., Wang Y., Zhai J., Liu J., Hong X.,
RA Cao X., Zhu J.-K., Gong Z.;
RT "ROR1/RPA2A, a putative replication protein A2, functions in epigenetic
RT gene silencing and in regulation of meristem development in Arabidopsis.";
RL Plant Cell 18:85-103(2006).
CC -!- FUNCTION: Component of the replication protein A complex (RPA) required
CC for DNA recombination, repair and replication. The activity of RPA is
CC mediated by single-stranded DNA binding and protein interactions.
CC Required fo cell division in meristems. Involved in the maintenance of
CC transcriptional epigenetic gene silencing (TGS) at specific loci
CC (including some transposons) by regulating histone H3 acetylation,
CC 'Lys-4' and 'Lys-9' methylation (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Heterotrimer of RPA1, RPA2 and RPA3 (canonical replication
CC protein A complex). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}. Note=Redistributes to
CC discrete nuclear foci upon DNA damage. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=Additional isoforms seem to exist.;
CC Name=1;
CC IsoId=Q8LFJ8-1; Sequence=Displayed;
CC -!- PTM: Phosphorylated in a cell-cycle-dependent manner (from the S phase
CC until mitosis). In response to DNA damage, recruited to DNA-repair
CC nuclear foci, as a hypophosphorylated form (By similarity).
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the replication factor A protein 2 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF26967.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC018363; AAF26967.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE73879.1; -; Genomic_DNA.
DR EMBL; AK117794; BAC42439.1; -; mRNA.
DR EMBL; BT024909; ABD91500.1; -; mRNA.
DR EMBL; AY084806; AAM61372.1; -; mRNA.
DR RefSeq; NP_566188.1; NM_111162.3. [Q8LFJ8-1]
DR AlphaFoldDB; Q8LFJ8; -.
DR BioGRID; 6520; 1.
DR STRING; 3702.AT3G02920.2; -.
DR iPTMnet; Q8LFJ8; -.
DR PRIDE; Q8LFJ8; -.
DR ProteomicsDB; 236893; -. [Q8LFJ8-1]
DR EnsemblPlants; AT3G02920.1; AT3G02920.1; AT3G02920. [Q8LFJ8-1]
DR GeneID; 821187; -.
DR Gramene; AT3G02920.1; AT3G02920.1; AT3G02920. [Q8LFJ8-1]
DR KEGG; ath:AT3G02920; -.
DR Araport; AT3G02920; -.
DR eggNOG; KOG3108; Eukaryota.
DR HOGENOM; CLU_051033_3_1_1; -.
DR InParanoid; Q8LFJ8; -.
DR OMA; EIASHFI; -.
DR OrthoDB; 924826at2759; -.
DR PhylomeDB; Q8LFJ8; -.
DR PRO; PR:Q8LFJ8; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8LFJ8; baseline and differential.
DR Genevisible; Q8LFJ8; AT.
DR GO; GO:0000781; C:chromosome, telomeric region; IBA:GO_Central.
DR GO; GO:0005662; C:DNA replication factor A complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0035861; C:site of double-strand break; IBA:GO_Central.
DR GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0006260; P:DNA replication; IBA:GO_Central.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IBA:GO_Central.
DR GO; GO:0006289; P:nucleotide-excision repair; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR040260; RFA2-like.
DR InterPro; IPR014646; Rfa2/RPA32.
DR InterPro; IPR014892; RPA_C.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR13989; PTHR13989; 1.
DR Pfam; PF08784; RPA_C; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR PIRSF; PIRSF036949; RPA32; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; DNA damage; DNA recombination; DNA repair;
KW DNA replication; DNA-binding; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..278
FT /note="Replication protein A 32 kDa subunit B"
FT /id="PRO_0000419969"
FT DNA_BIND 70..143
FT /note="OB"
SQ SEQUENCE 278 AA; 30957 MW; E3104284CB82A3EB CRC64;
MYGGDFDGNA AFAGGGFMPS QATTQAHESS SSLKNRDVRT LLPLTLKQLS SASTTGESNF
SIDGVDIKTV VIVGRISRME NRITQVDFVV DDGTGWVDCV RWCHARQETE EMEAVKLGMY
VRLHGHLKIF QGKRSVNVFS VRPVTDFNEI VHHFTECMYV HMYNTKLRGG SITQDTATPR
PQMPYSTMPT PAKPYQTGPS NQFPNQFNDS MHGVKQTVLN YLNQPMHIVS EAGVHCDIIA
RELRIPLLQV KEALEQLSND GCIYSTLDET CFKSTANA
