RFA2C_ORYSJ
ID RFA2C_ORYSJ Reviewed; 430 AA.
AC Q5Z8L1; A0A0P0X0N4; C7J489;
DT 26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT 26-JUN-2013, sequence version 2.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=Replication protein A 32 kDa subunit C;
DE Short=OsRPA32C;
DE AltName: Full=OsRPA32-3;
DE AltName: Full=Replication factor A protein 2C;
DE AltName: Full=Replication protein A 2C;
GN Name=RPA2C; Synonyms=RPA32C;
GN OrderedLocusNames=Os06g0693300, LOC_Os06g47830; ORFNames=P0550B04.15;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [5]
RP INTERACTION WITH RPA1C AND RPA3.
RX PubMed=16428324; DOI=10.1093/jb/mvj014;
RA Ishibashi T., Kimura S., Sakaguchi K.;
RT "A higher plant has three different types of RPA heterotrimeric complex.";
RL J. Biochem. 139:99-104(2006).
CC -!- FUNCTION: Component of the replication protein A complex (RPA) required
CC for DNA recombination, repair and replication. The activity of RPA is
CC mediated by single-stranded DNA binding and protein interactions (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Heterotrimer of RPA1, RPA2 and RPA3 (canonical replication
CC protein A complex) (By similarity). Interacts with RPA1C and RPA3.
CC {ECO:0000250, ECO:0000269|PubMed:16428324}.
CC -!- INTERACTION:
CC Q5Z8L1; Q65XV7: RPA1C; NbExp=4; IntAct=EBI-849544, EBI-849558;
CC Q5Z8L1; Q9SDK9: RPA3; NbExp=2; IntAct=EBI-849544, EBI-849521;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- PTM: Phosphorylated in a cell-cycle-dependent manner (from the S phase
CC until mitosis). In response to DNA damage, recruited to DNA-repair
CC nuclear foci, as a hypophosphorylated form (By similarity).
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the replication factor A protein 2 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD53865.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAH93699.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP003770; BAD53865.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008212; BAH93699.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014962; BAS99255.1; -; Genomic_DNA.
DR EMBL; AK102353; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015642557.1; XM_015787071.1.
DR AlphaFoldDB; Q5Z8L1; -.
DR SMR; Q5Z8L1; -.
DR ELM; Q5Z8L1; -.
DR IntAct; Q5Z8L1; 2.
DR STRING; 4530.OS06T0693300-01; -.
DR PaxDb; Q5Z8L1; -.
DR PRIDE; Q5Z8L1; -.
DR EnsemblPlants; Os06t0693300-01; Os06t0693300-01; Os06g0693300.
DR GeneID; 9266781; -.
DR Gramene; Os06t0693300-01; Os06t0693300-01; Os06g0693300.
DR KEGG; osa:9266781; -.
DR eggNOG; KOG3108; Eukaryota.
DR HOGENOM; CLU_051033_3_0_1; -.
DR InParanoid; Q5Z8L1; -.
DR OMA; QANQIFY; -.
DR OrthoDB; 924826at2759; -.
DR PlantReactome; R-OSA-9645850; Activation of pre-replication complex.
DR PlantReactome; R-OSA-9675782; Maturation.
DR PlantReactome; R-OSA-9675885; Lagging strand synthesis.
DR Proteomes; UP000000763; Chromosome 6.
DR Proteomes; UP000059680; Chromosome 6.
DR Genevisible; Q5Z8L1; OS.
DR GO; GO:0000781; C:chromosome, telomeric region; IBA:GO_Central.
DR GO; GO:0005662; C:DNA replication factor A complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0035861; C:site of double-strand break; IBA:GO_Central.
DR GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0006260; P:DNA replication; IBA:GO_Central.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IBA:GO_Central.
DR GO; GO:0006289; P:nucleotide-excision repair; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR040260; RFA2-like.
DR InterPro; IPR014892; RPA_C.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR13989; PTHR13989; 3.
DR Pfam; PF08784; RPA_C; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
PE 1: Evidence at protein level;
KW DNA damage; DNA recombination; DNA repair; DNA replication; DNA-binding;
KW Nucleus; Reference proteome.
FT CHAIN 1..430
FT /note="Replication protein A 32 kDa subunit C"
FT /id="PRO_0000422625"
FT DNA_BIND 86..160
FT /note="OB"
FT REGION 14..46
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 430 AA; 46328 MW; DFD46172046D146F CRC64;
MAAAASYFSG TALMPSQRSG APAPEYSAAG TGAAAAPSPS KPRDPRFSGC VPATVLHISR
SFAAALAADG GGDPVFSIDG VETTNVRVLG RVVSVVSRDT DVCFTLDDST GKIPLVRWIT
DQSDTRDTSY IQEGVYVKVQ VNLMGFQAKK QGLARSIRPI NNFNEVVLHF IECMHVHLES
VQSKMQRQLP PSVQTNEYTH VPSSGGVRDY QVHFTPQVNQ GLPPAVQTNT STYVPLLGGV
RDHQAHFAQV NQGQFSPAVQ ANTSTHLPFS GGVGEHQIHF TPKVNQGQFP PSVQTNTSAH
VPYSGGFREH QVHFTPPVNQ GQFPPAVQTN LYNHAASSGG VREQVHLTQA NQFSAYSSTG
GLQHDPQRMV LEALQQPDIL ALEHGAHVDE LVRRTGMPKA NIMGVVKHLA AAGFVYWTID
DNHVKSMCNG
