RFA3_YEAST
ID RFA3_YEAST Reviewed; 122 AA.
AC P26755; D6VW15;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1992, sequence version 1.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Replication factor A protein 3;
DE Short=RF-A;
DE AltName: Full=Replication protein A 13 kDa subunit;
GN Name=RFA3; OrderedLocusNames=YJL173C; ORFNames=J0506;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 8-22; 29-46 AND
RP 119-122.
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=1885001; DOI=10.1101/gad.5.9.1589;
RA Brill S.J., Stillman B.;
RT "Replication factor-A from Saccharomyces cerevisiae is encoded by three
RT essential genes coordinately expressed at S phase.";
RL Genes Dev. 5:1589-1600(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8221926; DOI=10.1007/bf00351791;
RA Loehning C., Rosenbaum C., Ciriacy M.;
RT "Isolation of the TYE2 gene reveals its identity to SWI3 encoding a general
RT transcription factor in Saccharomyces cerevisiae.";
RL Curr. Genet. 24:193-199(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: As part of the replication protein A (RPA/RP-A), a single-
CC stranded DNA-binding heterotrimeric complex, may play an essential role
CC in DNA replication, recombination and repair. Binds and stabilizes
CC single-stranded DNA intermediates, preventing complementary DNA
CC reannealing and recruiting different proteins involved in DNA
CC metabolism (By similarity). Stimulates the activity of a cognate strand
CC exchange protein (SEP1). {ECO:0000250}.
CC -!- SUBUNIT: Component of the heterotrimeric canonical replication protein
CC A complex (RPA). {ECO:0000250}.
CC -!- INTERACTION:
CC P26755; P22336: RFA1; NbExp=4; IntAct=EBI-14981, EBI-14971;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- PTM: The N-terminus is blocked.
CC -!- MISCELLANEOUS: Present with 4280 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the replication factor A protein 3 family.
CC {ECO:0000305}.
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DR EMBL; X59750; CAA42422.1; -; Genomic_DNA.
DR EMBL; X56792; CAA40110.1; -; Genomic_DNA.
DR EMBL; Z49448; CAA89468.1; -; Genomic_DNA.
DR EMBL; AY558541; AAS56867.1; -; Genomic_DNA.
DR EMBL; BK006943; DAA08631.1; -; Genomic_DNA.
DR PIR; C37281; C37281.
DR RefSeq; NP_012362.1; NM_001181606.1.
DR PDB; 6I52; EM; 4.70 A; A=1-122.
DR PDBsum; 6I52; -.
DR AlphaFoldDB; P26755; -.
DR SMR; P26755; -.
DR BioGRID; 33587; 171.
DR ComplexPortal; CPX-21; Replication protein A complex.
DR DIP; DIP-2109N; -.
DR IntAct; P26755; 48.
DR MINT; P26755; -.
DR STRING; 4932.YJL173C; -.
DR iPTMnet; P26755; -.
DR MaxQB; P26755; -.
DR PaxDb; P26755; -.
DR PRIDE; P26755; -.
DR EnsemblFungi; YJL173C_mRNA; YJL173C; YJL173C.
DR GeneID; 853266; -.
DR KEGG; sce:YJL173C; -.
DR SGD; S000003709; RFA3.
DR VEuPathDB; FungiDB:YJL173C; -.
DR eggNOG; ENOG502S18S; Eukaryota.
DR HOGENOM; CLU_2098758_0_0_1; -.
DR InParanoid; P26755; -.
DR OMA; YEFVCRS; -.
DR BioCyc; YEAST:G3O-31609-MON; -.
DR PRO; PR:P26755; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P26755; protein.
DR GO; GO:0000781; C:chromosome, telomeric region; IMP:SGD.
DR GO; GO:0000794; C:condensed nuclear chromosome; IDA:SGD.
DR GO; GO:0005662; C:DNA replication factor A complex; IDA:SGD.
DR GO; GO:0003690; F:double-stranded DNA binding; IDA:SGD.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:SGD.
DR GO; GO:0006281; P:DNA repair; IDA:ComplexPortal.
DR GO; GO:0006260; P:DNA replication; IDA:ComplexPortal.
DR GO; GO:0006265; P:DNA topological change; IDA:SGD.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IDA:SGD.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IGI:SGD.
DR GO; GO:0045184; P:establishment of protein localization; IPI:SGD.
DR GO; GO:0030491; P:heteroduplex formation; IDA:SGD.
DR GO; GO:0006312; P:mitotic recombination; IPI:SGD.
DR GO; GO:0006289; P:nucleotide-excision repair; IDA:SGD.
DR GO; GO:0016567; P:protein ubiquitination; IPI:SGD.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IPI:SGD.
DR GO; GO:0000723; P:telomere maintenance; IC:ComplexPortal.
DR GO; GO:0000722; P:telomere maintenance via recombination; IPI:SGD.
DR GO; GO:0007004; P:telomere maintenance via telomerase; IPI:SGD.
DR GO; GO:0010833; P:telomere maintenance via telomere lengthening; IMP:SGD.
DR InterPro; IPR013970; Rfa2.
DR InterPro; IPR016588; Rfp3_Saccharomycetes.
DR Pfam; PF08661; Rep_fac-A_3; 1.
DR PIRSF; PIRSF011773; Rep_factor-A_4; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; DNA replication; Nucleus;
KW Reference proteome.
FT CHAIN 1..122
FT /note="Replication factor A protein 3"
FT /id="PRO_0000097279"
SQ SEQUENCE 122 AA; 13816 MW; 5EC5D6FB56BAC008 CRC64;
MASETPRVDP TEISNVNAPV FRIIAQIKSQ PTESQLILQS PTISSKNGSE VEMITLNNIR
VSMNKTFEID SWYEFVCRNN DDGELGFLIL DAVLCKFKEN EDLSLNGVVA LQRLCKKYPE
IY
