ID   RFAB_ECOLI              Reviewed;         359 AA.
AC   P27127; Q2M7U2;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   24-NOV-2009, sequence version 2.
DT   03-AUG-2022, entry version 136.
DE   RecName: Full=Lipopolysaccharide 1,6-galactosyltransferase;
DE            EC=2.4.1.-;
DE   AltName: Full=UDP-D-galactose--(Glucosyl)lipopolysaccharide-alpha-1,3-D-galactosyltransferase;
GN   Name=rfaB; Synonyms=waaB; OrderedLocusNames=b3628, JW3603;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=1624461; DOI=10.1128/jb.174.14.4736-4745.1992;
RA   Pradel E., Parker C.T., Schnaitman C.A.;
RT   "Structures of the rfaB, rfaI, rfaJ, and rfaS genes of Escherichia coli K-
RT   12 and their roles in assembly of the lipopolysaccharide core.";
RL   J. Bacteriol. 174:4736-4745(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA   Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT   "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT   from 76.0 to 81.5 minutes.";
RL   Nucleic Acids Res. 22:2576-2586(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- FUNCTION: Adds a galactose goup to a glucose group of LPS.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; LPS core biosynthesis.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family.
CC       Glycosyltransferase 4 subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA24085.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=AAB18605.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=BAE77664.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; M80599; AAA24085.1; ALT_INIT; Genomic_DNA.
DR   EMBL; U00039; AAB18605.1; ALT_INIT; Genomic_DNA.
DR   EMBL; U00096; AAC76652.2; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77664.1; ALT_INIT; Genomic_DNA.
DR   PIR; B42982; B42982.
DR   RefSeq; NP_418085.2; NC_000913.3.
DR   RefSeq; WP_000683964.1; NZ_LN832404.1.
DR   AlphaFoldDB; P27127; -.
DR   SMR; P27127; -.
DR   BioGRID; 4263407; 262.
DR   DIP; DIP-10663N; -.
DR   IntAct; P27127; 1.
DR   STRING; 511145.b3628; -.
DR   CAZy; GT4; Glycosyltransferase Family 4.
DR   jPOST; P27127; -.
DR   PaxDb; P27127; -.
DR   PRIDE; P27127; -.
DR   EnsemblBacteria; AAC76652; AAC76652; b3628.
DR   EnsemblBacteria; BAE77664; BAE77664; BAE77664.
DR   GeneID; 948144; -.
DR   KEGG; ecj:JW3603; -.
DR   KEGG; eco:b3628; -.
DR   PATRIC; fig|1411691.4.peg.3078; -.
DR   EchoBASE; EB1326; -.
DR   eggNOG; COG0438; Bacteria.
DR   HOGENOM; CLU_009583_0_0_6; -.
DR   InParanoid; P27127; -.
DR   OMA; CVSGPGD; -.
DR   PhylomeDB; P27127; -.
DR   BioCyc; EcoCyc:EG11351-MON; -.
DR   BioCyc; MetaCyc:EG11351-MON; -.
DR   UniPathway; UPA00958; -.
DR   PRO; PR:P27127; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR   GO; GO:0008921; F:lipopolysaccharide-1,6-galactosyltransferase activity; IDA:EcoCyc.
DR   GO; GO:0009244; P:lipopolysaccharide core region biosynthetic process; IMP:EcoCyc.
DR   InterPro; IPR001296; Glyco_trans_1.
DR   InterPro; IPR028098; Glyco_trans_4-like_N.
DR   Pfam; PF13439; Glyco_transf_4; 1.
DR   Pfam; PF00534; Glycos_transf_1; 1.
PE   3: Inferred from homology;
KW   Glycosyltransferase; Lipopolysaccharide biosynthesis; Reference proteome;
KW   Transferase.
FT   CHAIN           1..359
FT                   /note="Lipopolysaccharide 1,6-galactosyltransferase"
FT                   /id="PRO_0000080303"
SQ   SEQUENCE   359 AA;  40826 MW;  191088896F4A45F4 CRC64;
     MKIAFIGEAV SGFGGMETVI RDVITTFRQQ HIQSEMFFFC RNDKMDKGWL EGIKYSCSFS
     NIRLGFLRRA KHIHALSKWL QEYQPDIVIC IDVISCLFAA KARKKSGIDM PVFSWPHFSL
     DHKKHAEYIT CADYHLAISS GIKQQMINRG VAESTINVIF NPVETKDSVI PAPEEGETAT
     FIYVGRMKFE GQKRVKDLLD GLSQAKGNWK LHVLGDGSDF EKCQAYGREL NIDDRIVWYG
     WQQYPWELVQ QDIEKVSALL LTSSFEGFPM TLLEALSWGI PCISADCVSG PADIIQPDVN
     GHLYQPGDIA GFVTLLNKYI AGEIHIEHEK IPASIDEFYQ SKYYDRLHKV IISAISRRK