RFAB_SALTY
ID RFAB_SALTY Reviewed; 359 AA.
AC Q06994;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1999, sequence version 2.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Lipopolysaccharide 1,6-galactosyltransferase;
DE EC=2.4.1.-;
DE AltName: Full=UDP-D-galactose--(Glucosyl)lipopolysaccharide-alpha-1,3-D-galactosyltransferase;
GN Name=rfaB; Synonyms=waaB; OrderedLocusNames=STM3719;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=LT2;
RX PubMed=9535865; DOI=10.1074/jbc.273.15.8849;
RA Heinrichs D.E., Monteiro M.A., Perry M.B., Whitfield C.;
RT "The assembly system for the lipopolysaccharide R2 core-type of Escherichia
RT coli is a hybrid of those found in Escherichia coli K-12 and Salmonella
RT enterica. Structure and function of the R2 WaaK and WaaL homologs.";
RL J. Biol. Chem. 273:8849-8859(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-70.
RC STRAIN=LT2;
RX PubMed=8444813; DOI=10.1128/jb.175.5.1524-1527.1993;
RA Klena J.D., Pradel E., Schnaitman C.A.;
RT "The rfaS gene, which is involved in production of a rough form of
RT lipopolysaccharide core in Escherichia coli K-12, is not present in the rfa
RT cluster of Salmonella typhimurium LT2.";
RL J. Bacteriol. 175:1524-1527(1993).
CC -!- FUNCTION: Adds a galactose goup to a glucose group of LPS.
CC -!- PATHWAY: Bacterial outer membrane biogenesis; LPS core biosynthesis.
CC -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family.
CC Glycosyltransferase 4 subfamily. {ECO:0000305}.
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DR EMBL; AF026386; AAC16413.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL22578.1; -; Genomic_DNA.
DR EMBL; S56361; AAB25552.2; -; Genomic_DNA.
DR PIR; C47074; C47074.
DR RefSeq; NP_462619.1; NC_003197.2.
DR RefSeq; WP_000683977.1; NC_003197.2.
DR PDB; 5N7Z; X-ray; 1.81 A; A=1-359.
DR PDB; 5N80; X-ray; 1.92 A; A=1-359.
DR PDB; 6Y6G; X-ray; 1.81 A; A=1-359.
DR PDB; 6Y6I; X-ray; 1.92 A; A=1-359.
DR PDBsum; 5N7Z; -.
DR PDBsum; 5N80; -.
DR PDBsum; 6Y6G; -.
DR PDBsum; 6Y6I; -.
DR AlphaFoldDB; Q06994; -.
DR SMR; Q06994; -.
DR STRING; 99287.STM3719; -.
DR CAZy; GT4; Glycosyltransferase Family 4.
DR PaxDb; Q06994; -.
DR DNASU; 1255243; -.
DR EnsemblBacteria; AAL22578; AAL22578; STM3719.
DR GeneID; 1255243; -.
DR KEGG; stm:STM3719; -.
DR PATRIC; fig|99287.12.peg.3933; -.
DR HOGENOM; CLU_009583_0_0_6; -.
DR OMA; CVSGPGD; -.
DR PhylomeDB; Q06994; -.
DR BioCyc; MetaCyc:STM3719-MON; -.
DR BioCyc; SENT99287:STM3719-MON; -.
DR UniPathway; UPA00958; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0009244; P:lipopolysaccharide core region biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR001296; Glyco_trans_1.
DR InterPro; IPR028098; Glyco_trans_4-like_N.
DR Pfam; PF13439; Glyco_transf_4; 1.
DR Pfam; PF00534; Glycos_transf_1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Glycosyltransferase; Lipopolysaccharide biosynthesis;
KW Reference proteome; Transferase.
FT CHAIN 1..359
FT /note="Lipopolysaccharide 1,6-galactosyltransferase"
FT /id="PRO_0000080304"
FT STRAND 3..8
FT /evidence="ECO:0007829|PDB:5N7Z"
FT STRAND 12..14
FT /evidence="ECO:0007829|PDB:5N7Z"
FT HELIX 15..28
FT /evidence="ECO:0007829|PDB:5N7Z"
FT STRAND 30..32
FT /evidence="ECO:0007829|PDB:5N80"
FT STRAND 36..41
FT /evidence="ECO:0007829|PDB:5N7Z"
FT STRAND 43..45
FT /evidence="ECO:0007829|PDB:5N7Z"
FT HELIX 50..52
FT /evidence="ECO:0007829|PDB:5N7Z"
FT STRAND 57..60
FT /evidence="ECO:0007829|PDB:5N7Z"
FT HELIX 67..84
FT /evidence="ECO:0007829|PDB:5N7Z"
FT STRAND 88..94
FT /evidence="ECO:0007829|PDB:5N7Z"
FT HELIX 95..108
FT /evidence="ECO:0007829|PDB:5N7Z"
FT STRAND 113..116
FT /evidence="ECO:0007829|PDB:5N7Z"
FT HELIX 122..124
FT /evidence="ECO:0007829|PDB:5N7Z"
FT HELIX 128..133
FT /evidence="ECO:0007829|PDB:5N7Z"
FT STRAND 134..141
FT /evidence="ECO:0007829|PDB:5N7Z"
FT HELIX 142..150
FT /evidence="ECO:0007829|PDB:5N7Z"
FT HELIX 155..157
FT /evidence="ECO:0007829|PDB:5N7Z"
FT STRAND 158..160
FT /evidence="ECO:0007829|PDB:5N7Z"
FT STRAND 181..187
FT /evidence="ECO:0007829|PDB:5N7Z"
FT TURN 193..195
FT /evidence="ECO:0007829|PDB:5N7Z"
FT HELIX 197..205
FT /evidence="ECO:0007829|PDB:5N7Z"
FT STRAND 211..216
FT /evidence="ECO:0007829|PDB:5N7Z"
FT HELIX 222..231
FT /evidence="ECO:0007829|PDB:5N7Z"
FT HELIX 235..237
FT /evidence="ECO:0007829|PDB:5N7Z"
FT STRAND 238..240
FT /evidence="ECO:0007829|PDB:5N7Z"
FT HELIX 247..254
FT /evidence="ECO:0007829|PDB:5N7Z"
FT STRAND 259..263
FT /evidence="ECO:0007829|PDB:5N7Z"
FT HELIX 272..279
FT /evidence="ECO:0007829|PDB:5N7Z"
FT STRAND 284..287
FT /evidence="ECO:0007829|PDB:5N7Z"
FT STRAND 290..292
FT /evidence="ECO:0007829|PDB:5N7Z"
FT HELIX 293..296
FT /evidence="ECO:0007829|PDB:5N7Z"
FT TURN 299..301
FT /evidence="ECO:0007829|PDB:5N7Z"
FT STRAND 302..306
FT /evidence="ECO:0007829|PDB:5N7Z"
FT HELIX 311..323
FT /evidence="ECO:0007829|PDB:5N7Z"
FT STRAND 324..326
FT /evidence="ECO:0007829|PDB:5N7Z"
FT HELIX 330..340
FT /evidence="ECO:0007829|PDB:5N7Z"
FT HELIX 342..354
FT /evidence="ECO:0007829|PDB:5N7Z"
SQ SEQUENCE 359 AA; 40890 MW; 5DBCFB1AA37AFB41 CRC64;
MKIAFIGEAV SGFGGMETVI SNVIHTFENS SPKINCEMFF FCRNDKMDKA WLKEIKYAQS
FSNIKLSFLR RAKHVYNFSQ WLKETSPDIV ICIDVISCLY ANKARKKSGK HFTIFSWPHF
SLDHKKHAEC ITYADYHLAI SSGIKEQIMA RGISAQDISV VYNPVSIKTV IVPPPERDKP
AVFLYVGRLK FEGQKRVKDL FDGLARTTGE WQLHIIGDGS DFEKCQAYSR ELGIEQRVIW
YGWQSAPWQV VQQKIKNVTA LLLTSAFEGF PMTLLEAMSY GIPCISSDCM SGPRDMIKPG
LNGELYTPGA IDDFVGHLNR VISGEVKYQH DIIPGTIERF YDVLYFKNFN NAIFSKLQK
