RFAF_ECOLI
ID RFAF_ECOLI Reviewed; 348 AA.
AC P37692; Q2M7T4;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=ADP-heptose--LPS heptosyltransferase 2;
DE EC=2.-.-.-;
DE AltName: Full=ADP-heptose--LPS heptosyltransferase II;
GN Name=rfaF; Synonyms=waaF; OrderedLocusNames=b3620, JW3595;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT from 76.0 to 81.5 minutes.";
RL Nucleic Acids Res. 22:2576-2586(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-51.
RC STRAIN=K12;
RX PubMed=2198271; DOI=10.1128/jb.172.8.4652-4660.1990;
RA Pegues J.C., Chen L., Gordon A.W., Ding L., Coleman W.G. Jr.;
RT "Cloning, expression, and characterization of the Escherichia coli K-12
RT rfaD gene.";
RL J. Bacteriol. 172:4652-4660(1990).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 52-348.
RC STRAIN=K12;
RX PubMed=8478319; DOI=10.1128/jb.175.9.2534-2540.1993;
RA Chen L., Coleman W.G. Jr.;
RT "Cloning and characterization of the Escherichia coli K-12 rfa-2 (rfaC)
RT gene, a gene required for lipopolysaccharide inner core synthesis.";
RL J. Bacteriol. 175:2534-2540(1993).
CC -!- PATHWAY: Bacterial outer membrane biogenesis; LPS core biosynthesis.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 9 family. {ECO:0000305}.
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DR EMBL; U00039; AAB18597.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76644.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77672.1; -; Genomic_DNA.
DR EMBL; M33577; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; X62530; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; S47841; S47841.
DR RefSeq; NP_418077.1; NC_000913.3.
DR RefSeq; WP_000699219.1; NZ_LN832404.1.
DR PDB; 1PSW; X-ray; 2.00 A; A=1-348.
DR PDBsum; 1PSW; -.
DR AlphaFoldDB; P37692; -.
DR SMR; P37692; -.
DR BioGRID; 4263306; 348.
DR DIP; DIP-10667N; -.
DR IntAct; P37692; 11.
DR STRING; 511145.b3620; -.
DR CAZy; GT9; Glycosyltransferase Family 9.
DR jPOST; P37692; -.
DR PaxDb; P37692; -.
DR PRIDE; P37692; -.
DR DNASU; 948135; -.
DR EnsemblBacteria; AAC76644; AAC76644; b3620.
DR EnsemblBacteria; BAE77672; BAE77672; BAE77672.
DR GeneID; 948135; -.
DR KEGG; ecj:JW3595; -.
DR KEGG; eco:b3620; -.
DR PATRIC; fig|1411691.4.peg.3086; -.
DR EchoBASE; EB2124; -.
DR eggNOG; COG0859; Bacteria.
DR HOGENOM; CLU_038371_7_0_6; -.
DR InParanoid; P37692; -.
DR OMA; GSAKRWP; -.
DR PhylomeDB; P37692; -.
DR BioCyc; EcoCyc:EG12210-MON; -.
DR BioCyc; MetaCyc:EG12210-MON; -.
DR BRENDA; 2.4.99.B7; 2026.
DR UniPathway; UPA00958; -.
DR EvolutionaryTrace; P37692; -.
DR PRO; PR:P37692; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0008713; F:ADP-heptose-lipopolysaccharide heptosyltransferase activity; IDA:CACAO.
DR GO; GO:0009244; P:lipopolysaccharide core region biosynthetic process; IMP:EcoCyc.
DR CDD; cd03789; GT9_LPS_heptosyltransferase; 1.
DR InterPro; IPR002201; Glyco_trans_9.
DR InterPro; IPR011910; RfaF.
DR Pfam; PF01075; Glyco_transf_9; 1.
DR TIGRFAMs; TIGR02195; heptsyl_trn_II; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Glycosyltransferase; Lipopolysaccharide biosynthesis;
KW Reference proteome; Transferase.
FT CHAIN 1..348
FT /note="ADP-heptose--LPS heptosyltransferase 2"
FT /id="PRO_0000207262"
FT CONFLICT 16
FT /note="Missing (in Ref. 4; M33577)"
FT /evidence="ECO:0000305"
FT CONFLICT 19..20
FT /note="SL -> DR (in Ref. 4; M33577)"
FT /evidence="ECO:0000305"
FT CONFLICT 105
FT /note="R -> S (in Ref. 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 151
FT /note="L -> R (in Ref. 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 159
FT /note="Q -> H (in Ref. 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 204..205
FT /note="EL -> DV (in Ref. 5)"
FT /evidence="ECO:0000305"
FT STRAND 2..6
FT /evidence="ECO:0007829|PDB:1PSW"
FT HELIX 11..27
FT /evidence="ECO:0007829|PDB:1PSW"
FT STRAND 32..37
FT /evidence="ECO:0007829|PDB:1PSW"
FT HELIX 39..41
FT /evidence="ECO:0007829|PDB:1PSW"
FT HELIX 42..45
FT /evidence="ECO:0007829|PDB:1PSW"
FT STRAND 51..56
FT /evidence="ECO:0007829|PDB:1PSW"
FT HELIX 66..75
FT /evidence="ECO:0007829|PDB:1PSW"
FT TURN 76..80
FT /evidence="ECO:0007829|PDB:1PSW"
FT STRAND 82..86
FT /evidence="ECO:0007829|PDB:1PSW"
FT HELIX 92..94
FT /evidence="ECO:0007829|PDB:1PSW"
FT HELIX 95..99
FT /evidence="ECO:0007829|PDB:1PSW"
FT STRAND 103..108
FT /evidence="ECO:0007829|PDB:1PSW"
FT TURN 110..112
FT /evidence="ECO:0007829|PDB:1PSW"
FT TURN 114..116
FT /evidence="ECO:0007829|PDB:1PSW"
FT STRAND 119..121
FT /evidence="ECO:0007829|PDB:1PSW"
FT TURN 125..127
FT /evidence="ECO:0007829|PDB:1PSW"
FT HELIX 131..137
FT /evidence="ECO:0007829|PDB:1PSW"
FT HELIX 142..144
FT /evidence="ECO:0007829|PDB:1PSW"
FT HELIX 148..150
FT /evidence="ECO:0007829|PDB:1PSW"
FT HELIX 164..173
FT /evidence="ECO:0007829|PDB:1PSW"
FT STRAND 178..180
FT /evidence="ECO:0007829|PDB:1PSW"
FT STRAND 182..186
FT /evidence="ECO:0007829|PDB:1PSW"
FT HELIX 193..195
FT /evidence="ECO:0007829|PDB:1PSW"
FT HELIX 199..211
FT /evidence="ECO:0007829|PDB:1PSW"
FT STRAND 215..218
FT /evidence="ECO:0007829|PDB:1PSW"
FT HELIX 222..224
FT /evidence="ECO:0007829|PDB:1PSW"
FT HELIX 225..232
FT /evidence="ECO:0007829|PDB:1PSW"
FT HELIX 237..240
FT /evidence="ECO:0007829|PDB:1PSW"
FT STRAND 243..245
FT /evidence="ECO:0007829|PDB:1PSW"
FT TURN 247..249
FT /evidence="ECO:0007829|PDB:1PSW"
FT HELIX 252..260
FT /evidence="ECO:0007829|PDB:1PSW"
FT STRAND 262..270
FT /evidence="ECO:0007829|PDB:1PSW"
FT HELIX 271..278
FT /evidence="ECO:0007829|PDB:1PSW"
FT STRAND 283..290
FT /evidence="ECO:0007829|PDB:1PSW"
FT STRAND 302..308
FT /evidence="ECO:0007829|PDB:1PSW"
FT HELIX 325..328
FT /evidence="ECO:0007829|PDB:1PSW"
FT HELIX 332..347
FT /evidence="ECO:0007829|PDB:1PSW"
SQ SEQUENCE 348 AA; 39042 MW; C5F57BC94B15BA6A CRC64;
MKILVIGPSW VGDMMMSQSL YRTLQARYPQ AIIDVMAPAW CRPLLSRMPE VNEAIPMPLG
HGALEIGERR KLGHSLREKR YDRAYVLPNS FKSALVPFFA GIPHRTGWRG EMRYGLLNDV
RVLDKEAWPL MVERYIALAY DKGIMRTAQD LPQPLLWPQL QVSEGEKSYT CNQFSLSSER
PMIGFCPGAE FGPAKRWPHY HYAELAKQLI DEGYQVVLFG SAKDHEAGNE ILAALNTEQQ
AWCRNLAGET QLDQAVILIA ACKAIVTNDS GLMHVAAALN RPLVALYGPS SPDFTPPLSH
KARVIRLITG YHKVRKGDAA EGYHQSLIDI TPQRVLEELN ALLLQEEA
