ID   RFAH_ECO57              Reviewed;         162 AA.
AC   P0AFW1; P26614;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Transcription antitermination protein RfaH {ECO:0000255|HAMAP-Rule:MF_00951};
GN   Name=rfaH {ECO:0000255|HAMAP-Rule:MF_00951}; Synonyms=hlyT, sfrB;
GN   OrderedLocusNames=Z5362, ECs4770;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Enhances distal genes transcription elongation in a
CC       specialized subset of operons that encode extracytoplasmic components.
CC       RfaH is recruited into a multi-component RNA polymerase complex by the
CC       ops element, which is a short conserved DNA sequence located downstream
CC       of the main promoter of these operons. Once bound, RfaH suppresses
CC       pausing and inhibits Rho-dependent and intrinsic termination at a
CC       subset of sites. Termination signals are bypassed, which allows
CC       complete synthesis of long RNA chains. {ECO:0000255|HAMAP-
CC       Rule:MF_00951}.
CC   -!- SUBUNIT: Interacts with both the nontemplate DNA and the RNA polymerase
CC       (RNAP). {ECO:0000255|HAMAP-Rule:MF_00951}.
CC   -!- SIMILARITY: Belongs to the RfaH family. {ECO:0000255|HAMAP-
CC       Rule:MF_00951}.
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DR   EMBL; AE005174; AAG59036.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB38193.1; -; Genomic_DNA.
DR   PIR; B91225; B91225.
DR   PIR; H86071; H86071.
DR   RefSeq; NP_312797.1; NC_002695.1.
DR   RefSeq; WP_001192396.1; NZ_SEKU01000026.1.
DR   AlphaFoldDB; P0AFW1; -.
DR   BMRB; P0AFW1; -.
DR   SMR; P0AFW1; -.
DR   STRING; 155864.EDL933_5162; -.
DR   EnsemblBacteria; AAG59036; AAG59036; Z5362.
DR   EnsemblBacteria; BAB38193; BAB38193; ECs_4770.
DR   GeneID; 66672252; -.
DR   GeneID; 915134; -.
DR   KEGG; ece:Z5362; -.
DR   KEGG; ecs:ECs_4770; -.
DR   PATRIC; fig|386585.9.peg.4979; -.
DR   eggNOG; COG0250; Bacteria.
DR   HOGENOM; CLU_067287_5_0_6; -.
DR   OMA; NQGVECF; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0001073; F:transcription antitermination factor activity, DNA binding; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.70.940; -; 1.
DR   HAMAP; MF_00951; RfaH; 1.
DR   InterPro; IPR006645; NGN_dom.
DR   InterPro; IPR036735; NGN_dom_sf.
DR   InterPro; IPR043425; NusG-like.
DR   InterPro; IPR010215; Transcription_antiterm_RfaH.
DR   PANTHER; PTHR30265; PTHR30265; 1.
DR   Pfam; PF02357; NusG; 1.
DR   SMART; SM00738; NGN; 1.
DR   SUPFAM; SSF82679; SSF82679; 1.
DR   TIGRFAMs; TIGR01955; RfaH; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Reference proteome; Transcription;
KW   Transcription antitermination; Transcription regulation.
FT   CHAIN           1..162
FT                   /note="Transcription antitermination protein RfaH"
FT                   /id="PRO_0000097282"
SQ   SEQUENCE   162 AA;  18340 MW;  2A298947EBB58C65 CRC64;
     MQSWYLLYCK RGQLQRAQEH LERQAVNCLA PMITLEKIVR GKRTAVSEPL FPNYLFVEFD
     PEVIHTTTIN ATRGVSHFVR FGASPAIVPS AVIHQLSVYK PKDIVDPATP YPGDKVIITE
     GAFEGFQAIF TEPDGEARSM LLLNLINKEI KHSVKNTEFR KL